Biotek 405 LS, Operator'S Manual

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40 | Chapter 3: Operation
Model Buffer Switching One Buffer
405 TS/LS 300 250
HT 300 250
Buffer Switching models have four buffer valves; otherwise, only one reagent bottle
may be connected to the washer. For Select models (with Cell Wash (CW)
capability), the recommended prime volume is the same with and without Buffer
Switching.
Model Quick Prime Prime step
Select 300 Main Low Flow
150 150
Manifold Prime
: 50 mL is recommended to clear air from the manifold only and to
wet the tips. This is recommended before running a protocol to correct fluid loss
due to evaporation.
Dead Volume
BioTek's recommended prime volumes are based on purity testing and measured
dead volumes. Generally, priming with three times the dead volume assures
purity.
Model Dead Volume
405 TS/LS 108 mL
HT 108 mL
Select 129 mL
For models with buffer switching tubing, dead volume is ± 2 mL additionally.
Optimize protocols to improve evacuation
When a wash protocol leaves too much residual fluid in the wells, optimize the
protocol with these recommendations:
l
Add a secondary aspiration to a wash cycle on page 70, including Final Aspirate ,
l
Decrease the aspirate Travel Rate ,
l
Add a Delay to the Aspirate and/or Final Aspirate step ,
l
Lower the aspirate height (Z-axis position).
405™ Microplate Washer LS