Biotek 405 LS, Operator'S Manual

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Predefined Protocols Listing | 43
Predefined Protocols Listing
LHC Users
: Folders named 4Buffers and 1Buffer contain customized protocols for
models with and without Buffer Switching, respectively.
Keypad Users : The protocol names onboard the washer do not include the W- prefix and
some protocols include the model name, e.g. 405HT or 405Select, when appropriate.
Maintenance Protocols
Daily
Maintenance
Description
W-DAY_
RINSE Simple one-step protocol to fully flush the system with water or reagent to
keep the manifold tubes clog-free. Defined for use with Buffer A; 500 mL
total volume.
W-
OVERNIGHT_
LOOP Protocol designed to keep the manifold in a wetted condition overnight or
for a long downtime period; manifold tubes are submerged in fluid for 4-
hour intervals between primes in this virtually endless loop. Defined to use
Buffer A.
W-RINSE_
AND_SOAK Identical to W-DAY_RINSE with one addition, the manifold tubes are
submerged and soaked for 5 minutes in the fluid.
Periodic Maintenance
W-Decontaminate
DECON (onboard)_
STEP1, STEP2,
STEP3 Two/three stage protocol to decontaminate the washer, first flushing
lines with disinfectant and then rinsing them with deionized or distilled
water. Buffer switching models flush and then rinse all four lines.
Manifold tubes are submerged and soaked for 20 minutes during each
pass. Prompts guide the user through the process.
W-LONG_
SHUTDOWN
PURGE_WITH_AIR
(onboard) Helps implement the routine recommended for preparing the
instrument for storage. This protocol includes prompts for running
disinfectant from Buffer A, then water from Buffer B, and lastly, air
through the system - remove bottle from Buffer C valve.
W-CLEAN
(LHC Only) Combines priming and AutoClean steps to clean and rinse the
manifold. For units with the Buffer Switching module it is defined to
obtain cleaning fluid from Buffer B, and rinse fluid from Buffer A. Units
without Buffer Switching are prompted to change fluids.
W-PRIME_250/300 Simple prime routine; defined for Buffer valve A only. For LHC only.
PRIME_ALL_
BUFFRS Consecutively primes each of the Buffer Switching valves beginning
with D. Designed for use in the annual instrument verification test of
the Buffer Switching module.
BioTek Instruments, Inc.