Biotek 405 LS, Operator'S Manual

Page: 97 / 240

Verify Performance | 77
l
Pre-check : the sensor verifies the standard plate carrier is present and level, and the
Verify Test Plate is on the carrier. All wells are aspirated to make sure they are dry.
Tests
conducted by the Verify level sensor:
1.
Aspirate Minimum Capability
: Post-aspirate, this test verifies the residual
volume is less than 250 µL/well.
2.
Dispense Minimum Volume
: Post-dispense, this test verifies that every well has
at least 125 µL/well.
3.
Dispense Mean Volume
: Calculates the mean dispense volume: dispense
accuracy of ±20% is expected.
4.
Dispense Volume %CV
: Calculates the dispense %CV and per-well deviation
from mean.
5.
Aspirate Rate
: Calculates the aspirate rate per well in mL/seconds.
When the manifold tubes are clog-free, it is expected that all five tests pass and
normal operation can be resumed. If any of the tests fail, the procedure stops and
displays the results in a plate map format to instantly identify the clogged
manifold tubes.
The Verify Process Flow
The Verify Test proceeds this way when not interrupted by a failed test:
1.
Pre-check
: The process begins with the pre-check described above.
2.
Dispense
: 300 µL/well dispensed to each well in the test plate.
3.
Aspirate
: All wells are evacuated.
4. Shake plate to better distribute remaining fluid in the plate and improve the
meniscus.
5.
Scan
: Level sensor scans plate to measure fluid: <250 µL/well is expected.
Generate Test 1 results.
6.
Dispense
: 250 µL/well dispensed to each well in the test plate.
7. Shake plate to distribute fluid in the plate and improve the meniscus.
8.
Scan
: Level sensor scans plate to measure fluid: ≥125 µL/well plus residual
from aspirate step is expected. Generate Test 2 results.
9. Evaluate data and generate Test 3 and Test 4 results.
10.
Aspirate
: All wells are evacuated at the fastest travel rate (6CW).
11. Shake plate to distribute fluid in the plate and improve the meniscus.
12.
Scan
: Level sensor scans plate to measure aspirate rate: ≥0.4 mL/sec is
expected. Generate Test 5 results.
BioTek Instruments, Inc.