Biotek 405 LS, Operator'S Manual

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Biomagnetic Separation - Magnetic Bead Assays | 93
bottoms and edges of the wells.
Every well is circled by 4 magnets.
Magnet Height Offset
When performing magnetic bead assays/biomagnetic separation, this offset setting
is a real time saver. It increases the height or Z-axis for all processing options to
accommodate the increased height of the plate when the magnet is installed.
This setting eliminates the need to modify individual protocol parameters to adjust
the dispense and aspirate heights and enables Quick Wash (without adjustments)
when the magnet is used. It applies the specified height value as an offset to all
relevant steps.
Custom or non-standard microplates and special adapters for labware may benefit from
this setting, too. If a vessel's height has been its only limitation to processing with the 405
LS, i.e. the vessel's geometry permits the manifold tips to successfully address its wells,
this setting can be used to simplify protocol development.
See
Determine Magnet Height Offset below
.
For the best results, measure the height of the plate you are using with the magnet.
BioTek has determined that Nunc flat bottom plates are about 3 mm taller than a similar
Corning plate, for example.
The "In use" checkbox/option lets you specify and retain a height setting. Set the
value once and then use the control to switch it on and off: fill the checkbox
(keypad users: say Yes) when using a magnet, empty the checkbox (keypad: No) to
disable the offset.
LHC Keypad
1. Tools>
Instrument Utilities> General
2. Fill the
In use
checkbox and set the Magnet
Adapter height to the correct offset.
3. Click the
Send
link.
1.
SETUP > MAGNET
2. Select
YES
.
3. Enter the desired offset
value and press Enter.
Determine Magnet Height Offset
Use a caliper for the best results or another measuring device.
BioTek Instruments, Inc.