Biotek 405 LS, Operator'S Manual

Page: 155 / 240

Replacement Procedure for Verify Test Plate | 135
Required Materials:
l
Precision digital balance with a capacity of 400 g and readability of 0.01 g resolution
l
200 mL Solution #3 : Dispense Precision solution. This volume is sufficient for two test
runs. See Washer Qualification Test Materials on page 155 .
l
The Excel spreadsheet provided on the operator's manual CD to perform the required
calculations: in the Verify Technology Spreadsheets folder, open
VerifyPlateReplacement.xls .
Prerequisites:
l
Make sure the replacement Verify Test Plate is clean and dry to begin this procedure.
l
Mix the dye solution, fill a buffer bottle attached to the washer, and fully prime the
system.
1. Perform Test Plate Alignment
LHC
1. Select
Tools>Instrument Utilities>Verify Manifold>Configure Utilities
.
2. Put the test plate on the carrier and click
Test Plate Alignment
.
This process updates configuration data needed to successfully run the Verify
routine.
2. Determine the Slope and Offset Volume Coefficients
n
This step is not mandatory but strongly recommended to ensure the most
reliable performance.
1. Put the Verify Test Plate on the balance and tare the balance.
2. Run the special procedure:
Touch screen LHC
1. Select
Instrument>Other>Verify
Technology
.
2. Select
Plate Replacement
(select this
button in the Sensor Calibration box)
and press
Start
.
1. Select
Tools>Instrument
Utilities>Verify
Manifold>Configure Utilities
.
2. Click the
Sensor Calibration
link.
The 405 LS will perform the first part of the Verify process.
3. At the first pausing message, weigh the plate and record the
Aspirate Weight
(W
A
) value.
BioTek Instruments, Inc.