Biotek 405 LS, Operator'S Manual

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Target Instrument Settings | 67
Choose the intensity that most closely matches your assay kit instructions.
l
Soak : allows buffer/dispense fluid to remain in wells for the specified duration, i.e.
processing is delayed:
l
Duration
: enter values to stop processing the plate for up to 1 hour (59
minutes, 59 seconds).
n
Soak is not the same as "submerge" the tips. You must define a prime step or
use the AutoPrime feature to soak the tips in the priming trough.
n
Soak is equivalent to incubating the plate at room temperature or delaying the
protocol.
n
Shaking and soaking the plate is also an option when defining a Wash step.
Pre-Dispense
Wash and Dispense Step option
Also called
Tip Priming
.
Valid volume ranges:
Manifold µL/tube Default value
96-tube 50-3000 50
192-tube 25-3000 25
About the Pre-Dispense Options
Enable the Pre-dispense options to condition or normalize the dispense tubes
before dispensing fluid. This is recommended for precise dispensing to mitigate
evaporation or any potential fluid imbalance at the ends of the tubes.
The pre-dispense options are not intended to fully prime the tubing, for example,
when changing fluids. Be sure to fully
Prime
the device to remove all air from the
tubing before commencing a dispense step. Use the Pre-dispense feature to quickly
prime the tubes or tips between runs.
When a Pre-dispense is enabled, the 405 LS moves the plate to the home position
to allow the manifold to dispense into the priming trough.
How to condition the tips before dispensing
l
In a stand-alone Dispense step: select the Advanced Options link and fill
the Pre-dispense checkbox;
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