Biotek 405 LS, Operator'S Manual

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142 | Chapter 4: Maintenance
5. Soak the tubing and manifold in the bleach or alcohol solution.
6. Wait 20 minutes. Rinse the tubing and manifold with DI or distilled water.
7. Use a clean, dry cloth to dry all wet surfaces of the tubes and manifold.
8. Reassemble the washer manifold, making sure that the o-rings/gaskets are in
place prior to reassembly.
9. Re-attach the mist shield.
10. Prime the system by running
W-DAY_RINSE
or a similar Maintenance or Prime
protocol. Watch for leaks. If fluid leaks out of the back of the instrument,
firmly seat the tubing. If fluid leaks from the manifold, try disassembling and
carefully reassembling.
11. Discard the used gloves and cloths using a biohazard trash bag and an
approved biohazard container.
12. Verify performance visually or by performing qualification tests.
Decontaminate the Vacuum Filtration System
First, disinfect the tubing, then, remove and soak the carrier:
Decontaminate the tubing:
Run the predefined protocol
QC_96/384_VAC30/10_TEST
(depending on your
instrument model) with disinfectant as the fluid supply to clean the tubing and
hardware used to perform filter plate assays.
1. Connect a supply bottle filled with 200 mL of bleach solution/disinfectant.
2. Insert the black vent plug into the vent port on the front of the carrier.
3. First prime the tubing.
4. Run
QC_96/384_VAC30/10_TEST
.
5. When the run is completed, replace the decontamination fluid supply with
deionized or distilled water and rerun it once or twice.
Decontaminate the vacuum filtration carrier:
1. Turn off and unplug the instrument.
2. Disconnect the tubing from the vacuum carrier.
3. Remove the carrier and soak it in the Decontamination (bleach) solution for 20
minutes.
405™ Microplate Washer LS