Washer Liquid Tests | 157
Test Solutions
Solution #1: Buffer Solution
Pipette 1 mL Tween 20
into 1 liter (1000
mL) of deionized or distilled water and
mix well.
or
Pipette 10 mL of BioTek Wetting Agent* into
1 liter of deionized or distilled water and
mix well.
* BioTek Solution #1 100X Concentrate Wetting Agent 125 mL (PN 7773002)
contains 10% Tween 20 in deionized water and 0.01% Sodium Azide as a
preservative.
SOLUTION #2: Residual Test Solution
Mix 100 mL of
Solution #1
with 0.050
grams of FD&C #1 blue dye.
or
Mix 90 mL of
Solution #1
with 10 mL
of BioTek Blue Test Dye*.
* BioTek Solution #2 10X Concentrate Blue Test Dye 125 mL (PN 7773001)
contains 5 grams per liter FD&C Blue #1, 0.1% Tween 20 in deionized water and
0.01% Sodium Azide as a preservative.
SOLUTION #3: Dispense Precision Solution
for 96-Tube manifolds
Mix 1180 mL of deionized or distilled water with 20 mL of
Solution #2
.
SOLUTION #4: Dispense Precision Solution
for 192-Tube manifolds
Mix 1420 mL of
Solution #1
with 20 mL of
Solution #2
.
Dispense Precision Test (96)
n
This test is designed for
96-Tube
manifolds. Find the Dispense Precision Test
(192) on page 161.
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Find a list of the supplies you will need at Materials on page 155.
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Save the plate!
When this test is complete, use the filled plate to perform the
Evacuation Efficiency Test.
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Keypad Users
: The protocol names onboard the washer do not include the
W- prefix and some protocols include the model name, e.g. 405HT or
405Select, when appropriate.
1. Fill a supply bottle with 2 liters of deionized or distilled water; Buffer Switching
units use buffer valve A. Run the
W-DAY_RINSE
protocol two or three times to
prime the fluid lines and manifold.
BioTek Instruments, Inc.
Summary of Contents for 405 LS
Page 1: ...Operator s Manual Microplate Washer 405 LS ...
Page 2: ......
Page 56: ...36 Chapter 2 Installation 405 Microplate Washer LS ...
Page 134: ...114 Chapter 3 Operation 405 Microplate Washer LS ...
Page 190: ...170 Chapter 5 Qualification 405 Microplate Washer LS ...
Page 210: ...190 Chapter 7 Troubleshooting 405 Microplate Washer LS ...
Page 238: ......
