Biotek 405 LS, Operator'S Manual

Page: 126 / 240

106 | Chapter 3: Operation
When performing magnetic bead assays/biomagnetic separation, this offset setting
is a real time saver. It increases the height or Z-axis for all processing options to
accommodate the increased height of the plate when the magnet is installed.
This setting eliminates the need to modify individual protocol parameters to adjust
the dispense and aspirate heights and enables Quick Wash (without adjustments)
when the magnet is used. It applies the specified height value as an offset to all
relevant steps.
Custom or non-standard microplates and special adapters for labware may benefit from
this setting, too. If a vessel's height has been its only limitation to processing with the 405
LS, i.e. the vessel's geometry permits the manifold tips to successfully address its wells,
this setting can be used to simplify protocol development.
See
Determine Magnet Height Offset on page 93
.
In use
- this checkbox lets you retain the height offset value while turning off its
application. After determining the offset, enter the
Height
value, fill the checkbox to
apply the Magnet Height Offset. Conversely, empty the checkbox to disable the
offset. LHC users: don't forget to click
Send
to apply the change.
Sensors Enabled
l
Important: The washer must be primed, tubes filled, prior to a run.
l
These sensors cannot detect the depletion of fluid in the supply vessel during a protocol
step. Fill the supply bottle(s) with sufficient fluid to fully prime the system and complete
the protocol.
Fluid Detection
: measures the fluid level at the beginning and completion of a
wash cycle, dispense and AutoClean step. An error before the run suggests
insufficient fluid to begin. An error at the end of the run may indicate that less
volume than specified was dispensed.
Flow Detection
: monitors fluid flow during the run and issues a warning if the
pump is interrupted. An error indicates a problem with the pump.
Filter Vacuum Detection
: built into the vacuum filtration module, this sensor
detects errors in the setup of the vacuum filtration system, including disconnected
tubing, loose bottle cap, missing filter plate, non-functioning internal vacuum
pump and extremely low vacuum. This sensor is disabled by default.
Plate Detection
: washers with Verify™ Technology offer this sensor to detect the
presence of a microplate on the plate carrier before every protocol run. BioStack
users: the sensor checks for the first plate in the stack only.
405™ Microplate Washer LS