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54 | Chapter 3: Operation

Using LHC to Control the 405™ Washer

BioTek's Liquid Handling Control (LHC) software offers another way to design
protocols and control the instrument. The washer must be attached to and
communicating with your personal computer (PC) for the LHC to function.

Predefined Protocols

BioTek provides predefined protocols for maintenance routines, instrument
qualification, and other purposes like serial dilutions.

Click the

Open

button and locate the

405 LS

folder to open a predefined

protocol. Learn how to

Customize the Predefined Protocols on page 41

.

Introducing the LHC Workspace

Instrument Settings

: Click the

Name

link and select your instrument.

405 Steps on the facing page

405™ Microplate Washer LS

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Summary of Contents for 405 LS

Page 1: ...Operator s Manual Microplate Washer 405 LS ...

Page 2: ......

Page 3: ...405 Microplate Washer LS Operator s Manual BioTek Instruments Inc January 2013 January 2013 Part Number 1171030 Revision B ...

Page 4: ...trademark of BioTek Instruments Inc Microsoft Internet Explorer Windows Windows 7 Windows Vista Windows XP and Excel are either registered trademarks or trademarks of Microsoft Corporation in the United States and or other countries All other trademarks are the property of their respective holders Restrictions and Liabilities Information in this document is subject to change and does not represent...

Page 5: ...ive 2002 96 EC Waste Electrical and Electronic Equipment xv Directive 98 79 EC In Vitro Diagnostics if labeled for this use xv Electromagnetic Interference and Susceptibility xv USA FCC CLASS A xv Canadian Department of Communications Class A xvi User Safety xvi Safety Symbols xvii Introduction 1 Introducing the 405 Microplate Washer LS 2 Instrument Models 3 BioStack Compatibility 3 Package Conten...

Page 6: ...ating Protocols Washing Aspirating and Dispensing Fluid 41 LHC Users Only Customize the Predefined Protocols 41 Predefined Protocols Listing 43 Predefined Sample Protocols 44 Introducing the 405 LS Keypad 46 Quick Wash Keypad only 47 Quick Prime Keypad only 48 Create or Edit a Protocol Keypad Only 48 How to name a protocol Keypad only 49 Define the Plate Type and Plate Map or Partial Plate 50 How ...

Page 7: ...ets 91 Ring Magnets 92 Magnet Height Offset 93 Determine Magnet Height Offset 93 Manifold Stop Screw Adjustment Kit 94 Vacuum Filtration for Filter Plate Assays 97 Install the side bracket 98 Set up the Vacuum Filtration module 99 Enable Vacuum Filtration 101 Create a Vacuum Filtration Protocol 101 Install Vacuum Filtration Plate Carrier 103 Control the Vacuum Level 104 Washer Settings 105 Change ...

Page 8: ...s and Supplies 138 Decontaminate Exterior Surfaces 139 Decontaminate Tubing and Manifold 140 Alternate Decontamination Procedure for Tubing and Manifold 141 Decontaminate the Vacuum Filtration System 142 Long Shutdown Procedure Prepare for Storage or Shipment 143 Storing the Instrument 144 Replace Components 144 Remove Change Wash Manifold 145 Replace the Vacuum Pump Fuse 145 Vacuum Filtration Car...

Page 9: ...Test 192 162 Evacuation Diagnostic Test 192 163 Vacuum Filtration Evacuation Efficiency Test 165 Qualify the Verify Technology Sensor 167 Troubleshooting 179 Troubleshooting 180 General Operation Troubleshooting 182 Washer Problems 183 Communication Errors 188 Error Codes 191 System Error Codes 192 405 LS LHC Software Error Codes 200 205 BioTek Instruments Inc Index ...

Page 10: ...tside the U S Fax 802 655 7941 E Mail customercare biotek com Service TAC Phone 800 242 4685 toll free in the U S 802 655 4740 outside the U S Fax 802 654 0638 E Mail tac biotek com European Coordination Center Authorized European Representative BioTek Instruments GmbH Kocherwaldstrasse 34 D 74177 Bad Friedrichshall Germany Internet www biotek de Phone 49 0 7136 9680 Fax 49 0 7136 968 111 E Mail i...

Page 11: ...lled dH2O or deionized DI water can be met by numerous water purification methods including MilliQ A minimum water purity of 2mOhm is expected Document Revision History Rev Date Changes A 5 2012 First issue B 1 2013 Added content about BioTek s Verify Technology including the Plate Detection sensor and new shipping hardware Added instructions for running the Verify test analyzing its test results ...

Page 12: ...line through BioTek s Customer Resource Center CRC at www biotek com or by calling 888 451 5171 or 802 655 4740 Repackaging and Shipping If you need to ship the instrument to BioTek for service or repair contact BioTek for a Return Materials Authorization RMA number and use the original packing materials Other forms of commercially available packaging are not recommended and can void the warranty ...

Page 13: ...ch may obstruct the carrier path and cause the instrument to produce an error Warning Liquids Avoid spilling liquids on the instrument fluid seepage into internal components creates a potential for shock hazard or instrument damage If a spill occurs while a program is running abort the program and turn the instrument off Wipe up all spills immediately Do not operate the instrument if internal comp...

Page 14: ...rsonnel Only qualified technical personnel should perform troubleshooting and service procedures on internal components Caution Spare Parts Only approved spare parts should be used for maintenance The use of unapproved spare parts and accessories may result in a loss of warranty and potentially impair instrument performance or cause damage to the instrument Caution Environmental Conditions Do not ...

Page 15: ...e instrument according to Directive 2002 96 EC on waste electrical and electronic equipment WEEE or local ordinances Caution Warranty Failure to follow preventive maintenance protocols may void the warranty Caution Shipping Hardware All shipping hardware e g shipping bracket etc must be removed before operating the instrument and reinstalled before repackaging the instrument for shipment Caution H...

Page 16: ...adiated Emissions and Line Conducted Emissions Verification of compliance was conducted to the limits and methods of EN 55011 CISPR 11 Class A In a domestic environment it may cause radio interference in which case you may need to mitigate the interference Immunity The system has been type tested by an independent accredited testing laboratory and found to meet the requirements of EN 61326 1 and E...

Page 17: ...ional Institute of Standards and Technology NIST EN 61010 2 101 Particular requirements for in vitro diagnostic IVD medical equipment Electromagnetic Interference and Susceptibility USA FCC CLASS A RADIO AND TELEVISION INTERFERENCE NOTE This equipment has been tested and found to comply with the limits for a Class A digital device pursuant to Part 15 of the FCC Rules These limits are designed to p...

Page 18: ...s numerique de la Class A prescrites dans le Reglement sur le brouillage radioelectrique edicte par le ministere des Communications du Canada User Safety This device has been type tested by an independent laboratory and found to meet the requirements of the following l Underwriters Laboratories UL 61010 1 Safety requirements for electrical equipment for measurement control and laboratory use Part ...

Page 19: ...ción vease los documentosincluidos Attenzione consultare la doc annessa Warning risk of electric shock Attention risque de choc électrique Gefährlicheelektrischeschlag Precaución riesgo de sacudidaeléctrica Attenzione rischiodiscossaelettrica Warning risk of crushing or pinching Attention risqued écrasement et pincement Warnen Gefahr des Zerquetschens und Klemmen Precaución riesgo del machacamient...

Page 20: ...léctricos y electrónicos Raccolta separata delle apparecchiature elettriche ed elettroniche Consult instructions for use Consulter la notice d emploi Gebrauchsanweisung beachten Consultar las instrucciones de uso Consultare le istruzioni per uso 405 Microplate Washer LS ...

Page 21: ...ribes the instrument s features and specifications and includes important contact information Introducing the 405 Microplate Washer LS 2 BioStack Compatibility 3 Package Contents 5 Optional Accessories 7 Physical Specifications 9 Performance Specifications 10 BioTek s Customer Resource Center 12 ...

Page 22: ...to aspirate and dispense fluid 2 Priming trough and ultrasonic bath when equipped Certain models support BioTek s patent pending Ultrasonic Advantage ultrasonic cleaning of the wash manifold 3 Microplate Plate Carrier Holds standard microplates for processing and accommodates a magnet for biomagnetic separation assays 4 Keypad One of two ways to control the instrument the other way is LHC 405 Micr...

Page 23: ... to four separate reagents for complex assay requirements and to automate maintenance routines S S models are equipped with BioTek s patent pending Ultrasonic Advantage feature a built in ultrasonic cleaner or sonicator for enhanced maintenance of the manifold tubes A stainless steel cleaning reservoir with an ultrasonic transducer bonded to its bottom is mounted on the washer Q Q models are equip...

Page 24: ...ator s Manual for instructions on configuring the 405 LS to run with the BioStack To help you get started See Operating with the BioStack on page 81 If you are interested in purchasing the BioStack contact your local BioTek dealer for more information or visit our website at www biotek com 405 Microplate Washer LS ...

Page 25: ...cleaning washer manifold aspirate tubes 7102108 Stylus for cleaning washer manifold tubes 2872304 Stylus for cleaning 192 tube dispense manifold 7102139 Shipping bracket model dependent 7102138 1242018 or 1242021 1242032 and 1242019 Hex wrench 9 64 48434 Spare fuses 5 46055 Verify Test Plate for Q models only 01588 Manifold Stop Screw Kit Required for Vacuum Filtration 384 Well PCR processing and ...

Page 26: ...les 2 1170534 Complete Waste System 115V 230V 10L Bottles including 7100582 Waste 10L bottle 1 4L bottle with sensor 7103024 Vacuum pump 115V 230V High Flow Vacuum Pump Systems 1170532 Complete Dispense High Flow Waste System 115V 230V 4L Bottles including 7100753 Waste 4L bottles 2 one with sensor 1170529 Dispense 4L bottles with filters 2 7100753 Complete High Flow Waste System 115V 230V 4L Bott...

Page 27: ...uum pump tubing Notes 1 Models with Buffer Switching receive a dispense system Only a waste system must be ordered separately 2 High flow waste system may be necessary for 384 well washing with buffers not containing surfactant 3 Direct Drain Waste System use restricted to 96 tube single manifold models i e 405 LS Optional Accessories n Part numbers and package contents are subject to change and v...

Page 28: ...gnet 7102216 Vacuum Filtration Kit Description PN 96 well Only 1170008 96 and 384 well 1170009 Verify Technology Accessories Description PN Verify Test Plate Replacement Kit 1240001 Miscellaneous Accessories Description PN 96 tube Manifold Kit Replacement kit for 405 Select to support 384 well non surfactant buffer washing Tube in Tube 1172046S 96 Tube 7 Degree Dual Manifold Upgrade Kit Additional...

Page 29: ...um Line Filter for Waste Tubing 48294 Vacuum Gauge Regulator For use with house vacuum 4030551 Silencing Muffler for Vacuum Pump For use with P N 7103024 pump 01113 Fluid filter for dispense bottles does not include stainless steel adaptor 1172031 01310 Physical Specifications Labware Microplates 96 well 384 well that comply with SBS microplate standards 1 2004 2 2004 3 2004 and 4 2004 Microstrips...

Page 30: ...spense tubes arranged in a 16 x 12 array can only process 384 well plates Waste bottle volume 4 10 or 20 liters depending on the accessory package 2 bottles one with sensor Supply bottle volume 2 4L or 10L bottles 4 bottles w Buffer Switching Performance Specifications Average Residual Volume Evacuation Efficiency 96 Tube Manifold Single and Dual Average residual volume in the microwells is 2 µL p...

Page 31: ...3 0 CV when dispensing 300 µL per well of deionized water with 0 1 Tween 20 with FD C 1 blue dye at rate 6 into a Costar 96 well flat bottomed plate The absorbance of the solution is read at 630 nm and 450 nm reference 192 Tube Manifold 4 0 CV when dispensing 80 µL per well of deionized water with 0 1 Tween 20 with FD C 1 blue dye at rate 7 into a Costar 384 well flat bottomed plate The absorbance...

Page 32: ...formation about your BioTek microplate instrumentation and software On the secure CRC website you can l Track orders l Access warranty information user manuals and software updates l Download technical and application information l Maintain equipment inventory product registration l Request service and technical support l View service history l And much more Register at https customer biotek com 4...

Page 33: ...stallation instructions Unpack and Inspect the Instrument 14 Remove the Shipping Hardware 14 Setting Up the 405 LS 18 Install Software Connect to Computer 25 Connect to Power 26 Define Instrument Settings 26 Verify Performance 29 Repacking the 405 Washer 32 ...

Page 34: ...nge for repair or replacement of your instrument immediately before the shipping related claim is settled 1 Unpack the boxes containing the instrument and other equipment l 405 Microplate Washer LS and accessories l Vacuum Pump and accessories l Vacuum Filtration Accessory Kit 2 Place all packing materials back into the shipping boxes for reuse if necessary Refer to the Package Contents on page 5 ...

Page 35: ...t mount it on the back panel on the studs provided n Direct Drain Waste System if you are using the direct drain waste method wait till later to mount the shipping bracket For direct drain an intermediate collection bottle is attached to the washer using the same studs as the shipping bracket See the Direct Drain Kit instructions 405 LS with Verify Technology Q Models Q Models have three shipping ...

Page 36: ...aside Follow instructions to set up the washer e g install the vacuum pump and tubing Then perform Step 4 below 4 When you re ready to power up the instrument plug it in and turn it on Release the latch on the left side of the Verify sensor unit and to lift it up to remove the third shipping bracket 5 For safe keeping put the cap screws and washers for the two thin brackets in the studs provided o...

Page 37: ... each other on the studs on the back 7 Store the brackets mount them on the back panel on the studs provided Beginning with the longest 3rd bracket layer the brackets on top of each other and use the longest screws to secure them in place BioTek Instruments Inc ...

Page 38: ...es up to 31 C decreasing linearly to 50 relative humidity at 40 C Install the Microplate Carrier n Make sure the serial number on the underside of the plate carrier matches the washer s serial number If the numbers do not match call BioTek TAC immediately 1 Line up the tab on the underside of the carrier with the slot on the carrier transport block 2 Put the two carrier rail guides onto the transp...

Page 39: ... of the instrument This allows the 405 LS to regulate the pump turning it on and off as specified by the protocol When using a standard pump rather than the high flow pump set the instrument s Vacuum Dissipation Delay to prevent the pump from drawing excess current and blowing the 5 amp fuse See Define Instrument Settings on page 26 n Note The waste tubes have colored bands that match similarly co...

Page 40: ...bands g Bottle with waste sensor to the vacuum pump 1 Locate the quick release caps shipped inside the waste bottles and attach the tubing to them as follows 2 Connect the waste bottles to each other using the shortest length of tubing matching the colored bands on the tubing to colored dots on the caps 3 Attach the waste sensor cable to the Waste Sensor port on the back of the washer 4 Attach the...

Page 41: ...e s caps are well sealed n BioTek strongly recommends installing the vacuum line filter to protect your vacuum pump Install the Vacuum Line Filter The optional vacuum line filter PN 48294 can be installed halfway between the last waste bottle overflow bottle and the vacuum pump To do this cut the tubing and insert the filter noting the direction of flow The flow arrow on the filter should point to...

Page 42: ... bottles and tubing 1 Remove the Quick Release Connector from its bag inside the supply bottle and connect it to the raw supply tubing provided l You may need to cut the tubing to the desired length 2 Snap the connector into the top of the supply bottle 405 Microplate Washer LS ...

Page 43: ...t release the Quick Connector from the bottle cap use a paper towel to sop up the few drops in the cap Then refill the bottle With Buffer Switching 4 Buffers 1 Place the four supply bottles on the same surface as the instrument 2 Connect the tubing from one of the supply bottles to the A port on the back of the washer 3 Repeat step 2 with the other three supply bottles for B C and D Buffers BioTek...

Page 44: ... port Final Check l Verify that the tubing was not crimped during installation l Ensure that there are no loose fittings or cable connections Attach the Mist Shield 1 Position the mist shield so the gaps align with the thumbscrews The top rests on the two rubber pads above the manifold Always lift the mist shield straight up not towards you when removing it 405 Microplate Washer LS ...

Page 45: ...e Internet turn on the instrument Let Windows automatically locate and install the necessary USB drivers follow the online instructions if applicable or open the link below to download the drivers l Virtual Com Port VCP drivers for all Windows operating systems are available at http www ftdichip com Drivers VCP htm l If the computer is NOT connected to the Internet install the drivers using the su...

Page 46: ...LS supports voltage in the range of 100 240 V at 50 60 Hz 1 Plug the power cable into the power cable socket in the rear panel of the 405 LS 2 Insert the three prong plug into an appropriate receptacle Define Instrument Settings LHC Users Only When using the LHC to control the 405 LS an important first step is defining your instrument s settings After installing the LHC you can use the desktop ico...

Page 47: ... Click Exit to return to the main screen 1 Press Setup Menu 2 g Select the arrow for more options then ADVANC 3 Select VACDIS 4 Use the number pad to set the Vacuum Dissipate Delay to match your waste container 1 second per liter 5 Press Main Menu upon completion Define Startup Preferences LHC users only You can save enormous time creating protocols by following these steps to define a New Protoco...

Page 48: ...er 2 Installation Define startup behavior 8 After completing the steps above select the Startup Options tab 9 Select the button for New Protocol 10 Click OK to save your new preferences 405 Microplate Washer LS ...

Page 49: ...roubleshooting on page 180 to determine its cause If the problem is something you can fix turn off the instrument fix the problem and then turn the instrument back on Otherwise contact BioTek s Technical Assistance Center The Qualification Chapter in the operator s manual provides Installation and Operational Qualification procedures to perform after the instrument is installed and before the inst...

Page 50: ...Verify Technology run the test Important Before running the test Make sure the 405 LS is primed and ready to run l Fully prime the tubing system e g run Day Rinse l If necessary empty waste vessel and tighten waste bottle cap Prepare to run the Verify test l Fill the supply vessel with at least 100 mL dH2O or DI water or buffer solution1 l Put the Verify Test Plate on the standard mag bead plate c...

Page 51: ...on loss 40 mL of fluid is dispensed l CV Threshold optionally change the CV to match your lab s standard for QC tests 5 15 The default value of 5 is recommended 2 Press Start to run the test 3 Assess the results Verify Results Do not discard the Verify Test Plate Reuse it for as long as possible See Replacement Procedure for Verify Test Plate on page 134 if it is damaged Contact BioTek TAC if any ...

Page 52: ...strument before returning it See Decontamination on page 138 Step 1 Install the shipping bracket All models Remove the shipping bracket s from the storage place on the back of the washer Verify Technology Q models With manifold in its home position install the longest shipping bracket that secures the Verify sensor unit Set aside the large manifold bracket and smaller sensor bracket for later inst...

Page 53: ...to the Verify unit bracket 5 Remove the plate carrier and put it in the accessories box 6 405 Touch Tape the cardboard protector on top of the touch screen Obtain an RMA number l Contact BioTek TAC to obtain a Return Materials Authorization number l Write RMA on the shipping box in large clear letters l And include the RMA number in the shipping address label BioTek Instruments Inc ATTN RMA xxxxx ...

Page 54: ...g hardware l Put the washer in the plastic bag l Seat the washer in the foam base in the inner box and slide it forward pushing the manifold into the foam pockets Step 3 Slide in the cardboard and foam shipping blocks 2 to cushion the washer on both sides 405 Microplate Washer LS ...

Page 55: ...ut the tray on top of the washer in the inner box Tape the inner box closed Step 5 Put the corner blocks on the inner shipping box and put it in the outer shipping box Tape the box closed In large letters write RMA on the outer box and attach the shipping address label including your assigned RMA number BioTek Instruments Inc ...

Page 56: ...36 Chapter 2 Installation 405 Microplate Washer LS ...

Page 57: ...ng LHC to Control the 405 Washer 54 LHC Protocols 56 LHC Protocols Explained 57 Target Instrument Settings 58 Verify Performance 72 Configure Verify Technology Utilities 79 Operating with the BioStack 81 Cell Wash 84 Biomagnetic Separation Magnetic Bead Assays 89 Manifold Stop Screw Adjustment Kit 94 Vacuum Filtration for Filter Plate Assays 97 Washer Settings 105 Adjust Utility 108 Plate Types an...

Page 58: ...eters like fluid volume and number of wash cycles and rely on default parameters for the more advanced options See Quick Wash Keypad only on page 47 l Run a Wash Protocol using either the keypad or the LHC you can run a wash protocol to wash a plate You can run a predefined protocol or define your own protocol to specify the optimal parameters for your assay See Running Predefined Protocols on pag...

Page 59: ...at are recycled over several days will grow algae bacteria molds or other undesirable organisms l Prime before dispensing Priming the tubing is the most critical factor in assuring optimal performance l Empty the waste bottles and firmly seat the bottles caps and quick release connectors To make sure fluid does not back up into the vacuum pump during operation keep the waste sensor cable installed...

Page 60: ...ect fluid loss due to evaporation Dead Volume BioTek s recommended prime volumes are based on purity testing and measured dead volumes Generally priming with three times the dead volume assures purity Model Dead Volume 405 TS LS 108 mL HT 108 mL Select 129 mL For models with buffer switching tubing dead volume is 2 mL additionally Optimize protocols to improve evacuation When a wash protocol leave...

Page 61: ...nd follow the prompts Creating Protocols Washing Aspirating and Dispensing Fluid In addition to the quick routines available from the keypad s main menu you can define and run protocols Protocols offer more parameters giving you the ability to fine tune instrument performance and perform more complex processing Keypad Control Find instructions for creating and modifying protocols using the keypad ...

Page 62: ... the protocol file With the 405 LS connected to and communicating with the host computer i e make sure the instrument is turned on and not busy 1 Click the Open button locate the 405 LS folder and click Open 2 Open the Maintenance or other folder and select the desired protocol 3 Change the COM port if necessary click Port and enter the correct value or select from the drop down list 4 Click Setti...

Page 63: ...CON onboard _ STEP1 STEP2 STEP3 Two three stage protocol to decontaminate the washer first flushing lines with disinfectant and then rinsing them with deionized or distilled water Buffer switching models flush and then rinse all four lines Manifold tubes are submerged and soaked for 20 minutes during each pass Prompts guide the user through the process W LONG_ SHUTDOWN PURGE_WITH_AIR onboard Helps...

Page 64: ...ard the washer do not include the W prefix and some protocols include the model name e g 405HT or 405Select when appropriate LHC users First open the folder that matches your washer model e g 405 Select You may need to customize the protocols as described on page 41 to match your instrument s settings Cell Wash W CELLWASH_ 96 Cell wash protocol designed to minimize cell layer disturbance in 96 wel...

Page 65: ...ek s Flat magnets Begins by soaking the plate for 1 minute to let the beads settle at the bottom of the wells then performs a 2 cycle wash with 30 second soaks between cycles Recommendation Determine Magnet Height Offset on page 93 and apply it for the plate you are using especially when using 384 well plates W Luminex_ MAG_384 W Luminex_ VAC_96 Vacuum filtration wash protocols for Luminex xMap po...

Page 66: ...th and control the BioStack l RUN to run a previously defined protocol Use the Options key to select a protocol or enter its number See Predefined Protocols Listing on page 43 o Turn On Turn Off the Vacuum Pump to drain the priming trough At the Quick menu l Press Shift 1 to turn on the vacuum pump l Press Shift 2 to turn off the vacuum pump l Use these key sequences to manually control the vacuum...

Page 67: ...e currently installed hardware and press Enter to select the desired plate l WASH 003 is the number of wash cycles one aspirate and dispense step per cycle Select CYCLE to change the number of cycles to perform l VOL 0300 uL shows the dispense volume per well in microliters To change the dispense volume use the arrow keys to move the cursor to the desired number position The cursor appears to unde...

Page 68: ...lize the dispense tips and remove any air from the manifold l VOL 0300 mL shows the dispense volume per well in milliliters To change the dispense volume use the arrow keys to move the cursor to the desired number position The cursor appears to underline a number 0010 When the correct position is selected use the number pad to enter the desired value Or press Options to increment the value Shift O...

Page 69: ...g the Soft keys to save or discard your inputs for the current step Press Main Menu to end the session at any time Then select RUN and select the protocol to run it How to name a protocol Keypad only NAME _ At the Name screen when you are creating or editing a protocol you can enter up to 16 alphanumeric characters to name the protocol l Press Shift the number key for A H or scroll through the alp...

Page 70: ...low Then proceed to the plate map screen To change the plate type l DEFINE mode When defining a protocol press Next or Previous to specify the plate type g g g g Sector 01 04 386 l The two line display changes to show a representation of the current plate type s sectors in the top line with each sector as a filled or empty square empty sectors will not be washed Press the Options button to toggle ...

Page 71: ...e sector mode when you are concerned about the fluid drying out before the procedure is completed To learn more See About Wash Processing Patterns on page 111 How to shake the plate These instructions are for keypad control The shake command is tied to the Soak option These instructions apply to soaking or incubating the plate at room temperature as well as shaking There are two ways to specify a ...

Page 72: ... protocol that shares some of the same protocol parameters and then editing the copy to meet your needs LHC users Open the protocol you want to copy and select File Save As Assign unique file and protocol names to it Consult the Help to learn more Keypad users 1 Select DEFINE at the main menu 2 Select Copy 3 Choose the type of protocol you want to copy 4 Use the Options key or enter the protocol n...

Page 73: ...ntroducing the 405 LS Keypad 53 1 Select DEFINE at the main menu 2 Select DELETE 3 Use the Options key or enter the protocol number to select it 4 Select Yes to delete the protocol BioTek Instruments Inc ...

Page 74: ...he LHC to function Predefined Protocols BioTek provides predefined protocols for maintenance routines instrument qualification and other purposes like serial dilutions Click the Open button and locate the 405 LS folder to open a predefined protocol Learn how to Customize the Predefined Protocols on page 41 Introducing the LHC Workspace Instrument Settings Click the Name link and select your instru...

Page 75: ...uccessful l Check the cabling make sure you re using a new undamaged BioTek supplied cable and it is properly inserted into the instrument s USB or serial port l Turn on the washer make sure the instrument is on and not busy processing a plate running AutoPrime or performing a system test for example l Retry contact BioTek TAC if you are still unable to establish communication between the instrume...

Page 76: ...5 LS validates a protocol to be run on a specific instrument n Recommended Before changing a predefined protocol select File Save As and give it a unique name This practice preserves the custom protocol in the case of a future upgrade File Location The LHC installs the protocols in the Windows Common Applications Data Folder l Windows XP C Documents and Settings All Users Application Data l Window...

Page 77: ... you must specify the settings The Instrument Settings stored in the protocol file include the COM port and configuration details like the type of manifold installed and other details that are critical to controlling the instrument The LHC keeps track of the last used COM port for an instrument type For example when an EL406 runs a protocol the LHC logs the COM port used and the next time an EL406...

Page 78: ...ocol Errors are not reported unless the steps cannot be performed Target Instrument Settings n For LHC users only Click the Settings link in the main workspace Actual Instrument or Simulated Instrument That is the question for the Target Instrument Settings dialog When the 405 LS is l connected to the computer it is best to Get the actual settings from it l not connected to the computer you must d...

Page 79: ...6 tube Single 96 well plates only 96 tube Double 96 well and 384 well plates 192 tube 384 well plates only l Buffer Switching when this external valve module is installed for automatically switching wash buffers reagents for either the washer or Syringe dispenser l Vacuum Filtration when the instrument is equipped with the special carrier for washing filter bottom microplates l Ultrasonic Advantag...

Page 80: ...and click the Shake Soak button 2 Define an adequate duration and intensity to sufficiently mix the fluids in the plate Leave the Soak option disabled when you only want to mix the plate contents i e perform a stand alone shake step Alternatively enable the option and set a duration to delay the process to allow the fluids to interact n Soak is not the same as submerge the tips You must define a p...

Page 81: ...ove the wells to determine the required adjustments 6 Use the radio buttons to select the Axis you want to adjust 7 Use the Direction buttons to move one step at a time Press the companion button to reverse direction To move multiple steps at a time use the numeric field next to the Go to now link Enter a positive or negative number of steps to move the device or plate carrier and click the link T...

Page 82: ...Shift key to simultaneously select multiple files 4 Optionally at the top left corner of the screen choose to Disable Editing of transferred protocols to lock the protocols from editing or deleting when they are onboard the instrument 5 Click the applicable Upload or Download button The LHC will confirm the transfer or prompt you for more information When the transfer is complete you can manipulat...

Page 83: ... and performs fastest Delay Amount of time the tubes stay at the aspirate height before lifting out of the wells Define a delay between 0 5000 ms Increasing the delay may improve evacuation of the wells 0 Positioning X and Y horizontal axes Z height vertical axis can be adjusted to improve performance Default Z axis for 384 well plates is 22 steps 2 for 384 well PCR plates Z 29 for 96 well plates ...

Page 84: ... until the specified volume has been dispensed to the wells The range is 10 to 350 µL well 10 OPTS Options PRE Pre wash options Pre dispense Bottom wash if applicable MIDCYC Mid cycle options Shake Soak Pre dispense between cycles POST Post wash option Final Aspirate FORM Format for washing 384 well plates Plate or Sector PRE Pre wash Actions performed before the wash cycles begin Pre dispense Qui...

Page 85: ...horizontal axes Z height vertical axis can be adjusted Repositioning the tubes to harder to reach areas of the wells may the improve results MIDCYC Between cycles Shake To mix the contents of the plate No Duration From one second to one hour 5 sec Intensity Intensity Hertz Slow 7 Medium 13 Fast 19 Medium Soak Delays wash for the duration to allow fluids in the plate to steep or incubate No Duratio...

Page 86: ... Parameters Select the action to perform l Move carrier to home position first Regardless of selection the plate carrier is moved home when the total time of the shake and soak durations exceeds 1 minute The vacuum pump is turned off in this scenario Moving the plate home prevents drops from contaminating the plate n Note The Shake Soak step is performed wherever the carrier is positioned In a mul...

Page 87: ...old µL tube Default value 96 tube 50 3000 50 192 tube 25 3000 25 About the Pre Dispense Options Enable the Pre dispense options to condition or normalize the dispense tubes before dispensing fluid This is recommended for precise dispensing to mitigate evaporation or any potential fluid imbalance at the ends of the tubes The pre dispense options are not intended to fully prime the tubing for exampl...

Page 88: ... select rate 1 or 2 to use the low flow tubing to dispense fluid slowly and gently to avoid damaging well contents Rate µL tube second 96 tube 192 tube Cell Wash 1 116 NA Cell Wash 2 134 NA 3 204 102 4 244 122 5 273 136 5 6 306 153 Default 7 325 162 5 8 352 176 9 375 187 5 10 391 195 5 11 418 209 Cell Wash flow rates direct fluid through special low flow tubing which is not compatible with the sin...

Page 89: ...ause a common objective is minimizing the residual left in the wells during a wash cycle You can enable disable or modify the parameters of the final aspiration step n Important info for Vacuum Filtration Assays Be sure to reset or disable the Final Aspiration step to match your assay requirements Edit the Wash step to modify the Final Aspiration LHC Users Keypad Users 1 Double click the step in t...

Page 90: ...econd aspiration to the Final Aspirate also for best evacuation results 4 Optionally adjust the position of the manifold as it addresses the wells 1 Edit the Wash step or stand alone Aspirate step Define Edit select protocol and if necessary press Options to scroll to the desired step 2 Select ASPIR and press Enter until Secondary Aspirate is offered And or select OPTS POST to add a second aspirat...

Page 91: ... designed for gentle washing while bottom wash is designed for vigorous washing Advanced Options Parameters Description Bottom Wash Height Z axis Position The distance between the bottom of the aspirate tubes and the carrier surface The valid range is 12 to 175 The value in mm is displayed on screen Horizontal X axis Position The left and right position of the dispense tubes when the carrier is be...

Page 92: ...he Verify Test Plate on the facing page Run the Verify test LHC 1 Select Tools Instrument Utilities Verify Manifold and click Run a new Test l Buffer Select the bottle to use if applicable l Prime optionally prime the manifold tubes to correct for evaporation loss 40 mL of fluid is dispensed l CV Threshold optionally change the CV to match your lab s standard for QC tests 5 15 The default value of...

Page 93: ...routine less frequently e g before new assay runs Replace the Verify Test Plate according to the prescribed procedure if it is damaged scratched warped etc For more details See About Verify Technology on page 76 Manage Test Results Immediately after a test is run its results are displayed The test data file is named with the date and time You can view previous results using the drop down list of t...

Page 94: ...ys put the plate on the carrier with well A1 in the back left corner of the carrier l Clean the plate after each use if you are using wash buffer to run the test l Fully dry the plate after each use and before putting it back in its foam lined box l Inspect the plate regularly for damage e g scratches chips warping l Replace the plate when it is damaged See Replacement Procedure for Verify Test Pl...

Page 95: ... pass and normal operation can be resumed Verify Test Corrective Action Corrective Action 1 Clean the Manifold begin with AutoClean Quick Clean and rerun If test fails again remove the manifold to clean it more thoroughly See AutoClean the Washer on page 125 2 If the test fails after the manifold has been cleaned BioTek Instruments Inc ...

Page 96: ... plates will be washed according to your specifications or it will identify any clogged manifold tubes that need cleaning Our Verify Technology can significantly decrease the need for routine instrument quality control using fluorescence or absorbance based dyes and a microplate reader as the test can be performed with dH2O DI water and most wash buffers by the washer itself You can run the Verify...

Page 97: ...format to instantly identify the clogged manifold tubes The Verify Process Flow The Verify Test proceeds this way when not interrupted by a failed test 1 Pre check The process begins with the pre check described above 2 Dispense 300 µL well dispensed to each well in the test plate 3 Aspirate All wells are evacuated 4 Shake plate to better distribute remaining fluid in the plate and improve the men...

Page 98: ... of the Verify sensor itself to ensure it is meeting its performance specifications Qualify the Verify Technology Sensor on page 167 The Verify measurement variability is determined this way CV Verify σ V Verify CV accuracy is determined from the sigma measurement error and mean volume measurement error The sensor s performance specification is σ measurement 9 μL also typically σ measurement 3 μL ...

Page 99: ...ere may be too many raw data files in the Raw Data folder Archive the data files i e save the files in a different folder to restore normal performance l Archive files Develop a schedule that compliments your current QC QA practices to regularly archive Verify test raw data files Use Windows Explorer to move data files xml and report files txt to archive folders Test Plate Alignment Procedure for ...

Page 100: ...Settings Do not change the slope or offset values unless specifically instructed to do so by BioTek Note Instructions in the Replacement Procedure for the Verify Test Plate may recommend changing these values 405 Microplate Washer LS ...

Page 101: ... not deliver a plate You must create a protocol to process plates using the BioStack l You can use the Quick Prime option This is recommended especially prior to processing plates to remove air from the tubing n Only one of the 405 LS s communication ports can be used at a time you can plug in either the USB cable to connect it to the PC or the serial cable to connect it to the BioStack but not to...

Page 102: ...are available upon request Contact BioTek customer service Technical Note Only one of the two communication ports COM port on the instrument can be used at a time They cannot be used simultaneously You can use USB to connect the 405 LS to the computer or the RS232 serial port to connect to a BioStack or similar robotic device But you cannot use both ports simultaneously i e make sure only one cabl...

Page 103: ...ct the instruments with the dedicated serial cable before attempting to align the gripper with the washer Begin by pressing Home to enable the alignment and step up and down buttons When the gripper appears to be correctly positioned Save the Position and proceed with the Verify procedure Press the previous button to exit the screen BioTek Instruments Inc ...

Page 104: ... assays that require the addition and removal of buffer solution without disrupting the cells in the wells of the microplate Cells are often dislodged when fluid is dispensed at too high a pressure and lost during subsequent aspiration of the fluid from the well unless counter measures are taken The 405 LS is equipped with a low flow fluid path that provides a cell wash alternative for cell based ...

Page 105: ... Cell Wash Strategies described below Adjust the Dispense Flow Rate Height and Position when defining the dispense step be sure to select one of the special Flow Rates that trigger use of the low flow tubing 1 or 2 CW Also reposition the dispense tubes to aim the fluid at the side of the wells to further minimize turbulence See Cell Wash Strategies below CW Dispense Manifold As a result of BioTek ...

Page 106: ...andard 96 well Corning Costar plate best results were achieved with these values Dispense Step Settings Z height 120 steps X horizontal position 45 Y horizontal position 0 Aspirate Step Settings Z 46 steps X 35 Y 0 Secondary Aspirate Z 80 steps X 45 Y 0 About 40 µL well residual fluid is retained using these values 405 Microplate Washer LS ...

Page 107: ...s the wells as desired l Optimal cell wash performance is achieved with BioTek s special CW Dispense Manifold CW Dispense Manifold is engraved on the top of these newer manifolds to make them easy to recognize If you do not have one of these manifolds you may need to experiment with the Washer Settings on page 105 the CW Control to improve the performance of your cellular assays Contact BioTek to ...

Page 108: ...he specified volume is dispensed For cell wash assays specify at least 10 µL well For small dispense volumes BioTek recommends setting the vacuum on volume to equal your dispense volume Refer also to application notes on the BioTek web site for more information www biotek com 405 Microplate Washer LS ...

Page 109: ...ces away from the magnets Credit cards tapes and disks can be erased in the presence of a magnetic field Bodily harm pinching of hands and skin can result if magnets are not handled correctly Maintain distance between two or more magnets Take advantage of the sample magnetic bead protocols shipped onboard the 405 LS Copy W Luminex_Mag_Flat_96 for example and modify the parameters to suit your assa...

Page 110: ...N 3 HOME the BioStack and Begin Realignment 4 Lower the claw until a 0 050 1 3 mm gap between the bottom of the plate and the top of the gripper fingers is achieved and save the gripper position 5 Put the microplate in the input stack and Verify the alignment Remember to realign the BioStack for non bead assays when applicable Perform Magnetic Bead Assays For the best results when performing bioma...

Page 111: ...disabled between cycles l Adjust the Aspirate position When using Flat Magnets below position the aspirate tubes near the sides of the wells X axis if possible to improve bead retention l As always before running assays we recommend testing new protocols using deionized or distilled water and a little Tween 20 with the desired microplate and a magnet installed Flat Magnets Use this information abo...

Page 112: ...the VP magnets to fine tune wash protocol settings 96 Ring Magnet 7102216 384 Ring Magnet 7102215 96 Ring Magnet PN 7102216 This magnetic bead separator uses 329 of VP s 52 MGO magnets 7094 Gauss The magnets are arranged around each well pulling the magnetic beads to the bottoms and edges of the wells Aspirate from the center of the well the default position when using this magnet 384 Ring Magnet ...

Page 113: ...ng with the 405 LS i e the vessel s geometry permits the manifold tips to successfully address its wells this setting can be used to simplify protocol development See Determine Magnet Height Offset below For the best results measure the height of the plate you are using with the magnet BioTek has determined that Nunc flat bottom plates are about 3 mm taller than a similar Corning plate for example...

Page 114: ...nd measure the plate and carrier combined 5 Subtract the plate and carrier measurement from the measurement value of the total combined elements This is the offset value to use Example 24 6 mm 96 well Corning microplate 96 well flat magnet plate carrier 22 7 mm 96 well Corning microplate and plate carrier 1 9 mm Magnet Height Offset Manifold Stop Screw Adjustment Kit For Magnetic Bead Assays and 3...

Page 115: ... easier to swap between them Microplate Carrier Kit Contents l Screw height measurement tool jig l Two stop screws with adjustable nuts and lock washer l 5 32 Allen hex wrench l Two wrenches 5 16 and 3 8 Remove the carrier to perform most of these steps but always perform the measurement steps with the plate carrier installed correctly on the 405 LS 1 In the carrier s empty hole insert the taller ...

Page 116: ...he two supplied wrenches tighten the bottom nut to secure the screw then tighten the top nut to fully compress the washer between them This will lock the nuts in place and allow you to easily remove replace the screw without affecting its height setting 6 Verify the height with the jig and repeat steps 3 6 if necessary Instrument Settings Try applying different Plate Clearance and Magnet Height Of...

Page 117: ...r assay requirements Recommendations for best performance Here are some guidelines to achieve the best performance of your filter plate assays l Do not use dry filter plates If dry plates are required by the assay kit instructions turn off the Vacuum Filtration sensor to avoid process interruptions l Shake the plate to suspend the beads before aspiration Enable the wash cycle option to shake the p...

Page 118: ...mance in tests at BioTek were seen when the pressure was set to 2 5 inHg Install the side bracket Install the bracket on the right side of the washer to hold the vacuum filtration tubing used to evacuate the filter plate Locate the screws shipped with the bracket Required Philips screwdriver 1 Locate the two empty holes on the right side of the washer and line up the bracket over the holes 2 Inser...

Page 119: ...t of the control module It is a good guide for this step Three lengths of tubing are supplied to set up the control module Connect the tubing to the control module and to the intermediate waste bottle cap Tubing Control Module Waste Bottle Cap 1 Dual tubing connected by Luer fitting approx 15 38 1 cm Intermediate Waste Out Smallest nipple with long interior tube 2 Long single tube approx 15 38 1 c...

Page 120: ...ule 2 Connect the tubing with the Luer fitting to the port labeled Intermediate Waste Out and to the smallest nipple on the waste bottle cap 3 Connect the longest single tube to the port labeled Intermediate Vacuum Supply and to the nipple without a tube inside the bottle 4 Connect the shortest tube to the port labeled Intermediate Waste In and to the waste bottle cap nipple with the wide split tu...

Page 121: ...her Connect the supplied cable from the round port labeled To Filtration Valve Control on the control module to the corresponding Vacuum Filtration Control port on the back of the instrument Enable Vacuum Filtration LHC 405 LS Keypad 1 Select Tools Instrument Utilities Washer 2 Select installed for the Vacuum Filtration Module 1 Press Setup Menu 2 Select g ADVANC VACFIL 3 Select Yes for Vacuum Fil...

Page 122: ...d for vacuum filtration 8 Experiment with other parameters like number of cycles to optimize the protocol for your assay 7 Fill the checkbox for Shake Soak and specify the shake duration Experiment with different settings to determine the optimal parameters to meet your goals n Important Be sure to change the Plate Carrier setting to match the installed carrier Remember to specify the type of aspi...

Page 123: ...on Carrier 4 Tuck the rigid connector into the bracket you installed on the side of the instrument 5 Change the instrument setting LHC Keypad 1 Select Tools Instrument Utilities 2 For Plate Carrier Selection select Vacuum Filtration 1 Press Setup Menu MORE CARR 2 Select VAC Note Make sure Vacuum Filtration is enabled to select the special plate carrier Setup Menu g ADVANC VACFIL Important Always s...

Page 124: ...Level mmHg kPa No plug 0 067 1 70 mm Lowest 32 4 3 Beige with hole 0 047 1 19 mm Low 118 15 7 Gray 0 032 0 81 mm Medium 187 24 9 Black 0 020 0 51 mm High 382 50 9 Beige solid 0 00 Highest 567 75 5 Leave the vent open for the least amount of vacuum Insert one of the plugs to increase vacuum n Vacuum pressure is affected by several factors like relative humidity barometric pressure and mechanical to...

Page 125: ...c Black 7 1 sec Beige 6 4 sec Millipore 96 well 1 2 µM filter plate 200 µL well sample volume Beige w hole 6 2 sec Gray 4 7 sec Black 4 6 sec Beige 3 9 sec Millipore 384 well 1 2 µM filter plate 100 µL well sample volume Beige w hole 8 9 sec Gray 6 5 sec Black 6 0 sec Beige 4 7 sec Washer Settings Touch screen Instrument Options LHC users Tools Instrument Utilities Washer Manifold Selection After ...

Page 126: ...ckbox to disable the offset LHC users don t forget to click Send to apply the change Sensors Enabled l Important The washer must be primed tubes filled prior to a run l These sensors cannot detect the depletion of fluid in the supply vessel during a protocol step Fill the supply bottle s with sufficient fluid to fully prime the system and complete the protocol Fluid Detection measures the fluid le...

Page 127: ... disabling this setting Additionally a minor adjustment to the duration setting may improve the performance of certain instruments Service Functions Position the wash manifold to install the shipping bracket prior to packing up the instrument click the link and install the manifold shipping bracket Change the Washer Manifold Setting Keypad The instrument s onboard settings must match the installed...

Page 128: ...ffset affects the travel height as well as dispense and aspirate heights The Magnet Height Offset increases the respective values of all height parameters Generally it is best to keep the default setting for Plate Clearance when using the Magnet Height Offset setting To change the setting using the keypad 1 Press Setup Menu button in center of keypad 2 Select g and ADVANC then PLTCLR 3 Enter an of...

Page 129: ...orrect values are defined in the protocol you ll have a big mess on your hands Positional Limits The allowable ranges for positioning the devices for operation X Y Z Washer 60 to 60 steps 2 74 mm offset 40 to 40 2 96 mm offset 1 to 210 steps 26 68 mm max Run the Adjust Utility Using the Keypad This page describes how to run the Adjust Utility using the keypad rather than the LHC Find LHC instructi...

Page 130: ...ype of hardware installed on the instrument e g manifold type the 405 LS can process several plate types The default parameters for wash and dispense steps represent the optimal positioning of the hardware for the plate type And the aspirate and dispense heights and horizontal positions can be adjusted when necessary for special situations and to optimize assay performance l Review the Plate Types...

Page 131: ...ight physical measurement Default Dispense Height plate height flange height 1 0 mm Travel Height plate height flange height Plate Clearance n If Dispense Height Travel Height greater than the travel height is changed to match the dispense height About Wash Processing Patterns When using a 96 tube manifold to process a 96 well plate all wells are processed simultaneously To process 384 well plates...

Page 132: ...e sector or sectors you want to process n Keypad users see Define the Plate Type and Plate Map or Partial Plate on page 50 for instructions Checkmarks show the sectors to be processed Numbers show potential sectors that are currently unselected and will not be washed The 96 tube manifold processes the plate in four sectors A B C D 192 tube wash pattern for 384 well plate To process a partial plate...

Page 133: ... Type and Plate Map or Partial Plate on page 50 for instructions Checkmarks show the sectors to be processed Numbers show potential sectors that are currently unselected and will not be washed The 192 tube manifold processes the plate in two sectors A B BioTek Instruments Inc ...

Page 134: ...114 Chapter 3 Operation 405 Microplate Washer LS ...

Page 135: ... Clean the Vacuum Filtration Carrier 129 Clean the exterior surfaces and mist shield 130 Clean the Buffer Bottle Filter 130 Remove and clean the washer manifold 131 Maintaining the Vacuum Filtration System 133 Clean the Vacuum Filtration Carrier 133 Maintain the Verify Technology Components 133 Clean the Verify Level Sensor 133 Replacement Procedure for Verify Test Plate 134 Volume coefficients Sl...

Page 136: ...apter 4 Maintenance Storing the Instrument 144 Replace Components 144 Remove Change Wash Manifold 145 Replace the Vacuum Pump Fuse 145 Vacuum Filtration Carrier Replace the Gasket 146 405 Microplate Washer LS ...

Page 137: ...s dispense and aspirate tubes can clog quickly and must be rinsed frequently and cleaned regularly Run AutoClean ultrasonic cleaning regularly when available Otherwise Remove and clean the washer manifold on page 131 o If the washer will be idle for several hours or days at a time soak the tubes to keep them in a wetted state Enable AutoPrime if the washer is idle for more than 3 hours o Wash solu...

Page 138: ...118 Chapter 4 Maintenance numerous water purification methods including MilliQ A minimum water purity of 2mOhm is expected 405 Microplate Washer LS ...

Page 139: ..._LOOP ü Run W RINSE_AND_SOAK ü Run Verify clog detection test ü Run AutoClean ü ü Components Remove protein residuals and fungi growth if necessary ü ü ü Check empty waste bottles ü ü Clean bottles ü ü Clean plate carrier system ü ü Clean washer manifold ü ü Clean aspirate and dispense tubes ü ü Clean exterior surfaces and mist shield ü Clean fluid inlet filter ü ü Clean vacuum filtration system ü...

Page 140: ...ks Frequency Daily Overnight Multi Day Weekly Periodic Monthly Before storage shipment Prepare for Storage or Shipment Run W LONG_SHUTDOWN ü Replace Repair Components Replace the Verify Test Plate As Needed 405 Microplate Washer LS ...

Page 141: ...nt rinse solution and that the waste bottles are empty before running the protocols Also see the additional maintenance procedures required when dispensing protein solutions Removing Protein Residuals and Fungi Growth on page 124 AutoPrime AutoPrime automatically conditions the dispense tubes priming them with the specified volume after a user specified amount of idle time See AutoPrime on next pa...

Page 142: ...lly primes the tubing whenever the instrument is idle for a specified time Keeping the tubes wet prevents clogging and mitigates fluid evaporation at the tips AutoPrime s submerge feature lets you soak the tubes for extended periods which is an effective maintenance option Specify the Interval and AutoPrime Parameters AutoPrime runs when the instrument has been idle for a specified interval To set...

Page 143: ...setup instructions AutoPrime Parameters Washer AutoPrime Interval 15 minutes Device Washer AutoPrime Yes Enabled Volume 90 mL Rate 09 Buffer Any Submerge Duration 120 minutes 2 hours Turn on AutoPrime for the Washer l Learn about AutoPrime if you haven t already done so n Keypad users Select UTIL at the main menu then AUTPRM and specify parameters 1 Select Tools Instrument Utilities AutoPrime 2 Fi...

Page 144: ...enu quick menu or run completion message is displayed Removing Protein Residuals and Fungi Growth Important Solutions containing proteins such as bovine serum albumin BSA will compromise the 405 LS s performance over time unless a strict maintenance regime is adhered to Do not use isopropyl alcohol to flush out BSA When using protein solutions or similar fluids BioTek recommends performing the fol...

Page 145: ...s to fill a four liter supply bottle Connect the bottle to the washer s Buffer valve A Connect a bottle of deionized or distilled water to Buffer valve B to rinse the tubing 2 Run the DECONTAMINATE protocol as appropriate 3 LHC users Respond to the Delay message Connect a bottle of water leave the detergent bottle connected and when ready press Continue 4 When the protocol is completed connect a b...

Page 146: ...tainless steel reservoir with an ultrasonic transducer bonded to the bottom of the reservoir The reservoir is mounted under the washer manifold and also functions as the priming trough n Do not try to remove the ultrasonic cleaner Only BioTek authorized service personnel should remove the ultrasonic cleaner for maintenance or repair While the program is running the ultrasonic cleaner will pulse on...

Page 147: ...utoClean protocol Create an AutoClean protocol for regular use Make sure it begins by priming the tubing with the cleaning fluid Approximately 93 mL of fluid fills the reservoir during each run General Recommendation Run AutoClean for 1 hour Follow with a full prime using deionized water to remove the detergent from the system and or with a wash buffer to leave the instrument primed and ready for ...

Page 148: ...nance procedures provided in this manual the requirement to use distilled dH2O or deionized DI water can be met by numerous water purification methods including MilliQ A minimum water purity of 2mOhm is expected Important l Do not immerse the instrument spray it with liquid or use a wet cloth on it l Do not allow the cleaning solution to run into the interior of the instrument If this happens cont...

Page 149: ...er transport rail or glide strips some buildup may occur and prevent the microplate from seating correctly on the carrier This can interfere with plate transport Weekly cleaning is recommended 1 Turn the instrument off 2 Lift the carrier up and off the transport rail 3 Clean the carrier rails and glide strips using mild detergent and hot water 70 isopropyl alcohol or ethanol Clean the priming trou...

Page 150: ...t side and lift the level sense unit above the wash manifold to gain access to all sides of the wand Gently wipe off the wand 5 If detergent was used wipe all surfaces with a cloth moistened with water 6 Use a clean dry cloth to dry all wet surfaces Clean the Buffer Bottle Filter Periodically clean the buffer bottle filter PN 01310 n Perform this task when the buffer bottle is empty or over a sink...

Page 151: ...m the fluid or connect an empty supply bottle to the 405 LS and run a prime protocol e g RINSE_ _SOAK until the tubing is empty 2 Turn off the instrument disconnect the power cable and remove the mist shield n Dual manifolds Hold the two manifolds together as a single unit when removing and reinstalling Have a towel handy to mop up drips from the manifold BioTek Instruments Inc ...

Page 152: ...ed water Check to see if water comes out of all dispense and aspirate tubes If not soak the manifold in hot soapy water and repeat 7 When satisfied reassemble the manifold and end plates 8 Install the mist shield 9 Reconnect the power cable and turn on the instrument 10 Prime the system with deionized water by running DAY_RINSE or a similar Maintenance or Prime protocol Watch for leaks If fluid le...

Page 153: ...erify Technology Q models performing as expected l Keep the Verify Test Plate clean Remove any salt deposits or other contaminants l Regularly inspect the plate for damage l Inspect the plate at least annually and replace it when necessary Replacement Procedure for Verify Test Plate on next page l Clean the level sensor at least annually or as needed Clean the Verify Level Sensor below Clean the V...

Page 154: ...oft lint free towel or allow to air dry 6 When the collimator is completely dry reinstall it Snap the collimator back in place on the level sensor Replacement Procedure for Verify Test Plate Before it leaves the factory BioTek calibrates the 405 LS to use a specific Verify Test Plate That test plate is labeled with 405 LS s serial number When a replacement plate is needed follow these instructions...

Page 155: ...t LHC 1 Select Tools Instrument Utilities Verify Manifold Configure Utilities 2 Put the test plate on the carrier and click Test Plate Alignment This process updates configuration data needed to successfully run the Verify routine 2 Determine the Slope and Offset Volume Coefficients n This step is not mandatory but strongly recommended to ensure the most reliable performance 1 Put the Verify Test ...

Page 156: ...ided o Select Ctrl A and Ctrl C o Select the From405 tab and paste Ctrl V all the data into the worksheet beginning at cell A1 The slope and offset are calculated using these formulas Slope calculation Slope W W D D 9600 D A D A Offset calculation Offset Slope D W D 0 096 D o D D is the Dispense Scan Mean value o D A is the Aspirate Scan Mean value reported in the date and time stamped txt file 8 ...

Page 157: ...y to work with a specific Verify Test Plate These volume coefficients have been gravimetrically determined and set for your washer test plate combination Important Do not change these values unless specifically instructed to do so by BioTek as when replacing the test plate for example Note default values are Slope 0 6 and Offset 200 0 BioTek Instruments Inc ...

Page 158: ...ese solutions and methods Each laboratory must ensure that decontamination procedures are adequate for the biohazards they handle Warning Internal Voltage Turn off and unplug the instrument for all decontamination operations n Do not immerse the instrument spray it with liquid or use a wet cloth Do not allow the cleaning solution to run into the interior of the instrument If this happens contact t...

Page 159: ...1 20 dilution Household bleach is typically 5 NaClO in this case prepare a 1 10 dilution The bleach solution is caustic wear gloves and eye protection when handling 1 Turn off the instrument and disconnect the power cord Empty the waste bottle 2 Prepare an aqueous solution of 0 5 sodium hypochlorite NaClO or bleach As an alternative 70 isopropyl alcohol or 70 ethanol may be used if the effects of ...

Page 160: ...luid are installed onboard the instrument and on the host computer during installation of the LHC o DECONTAMINATE for the washer When storing or shipping the instrument the LONG_SHUTDOWN procedure on page 143 primes and soaks the instrument and ends by pushing air through the system The parameters can be edited for optimum cleaning For example consider using ethanol instead of air to complete the ...

Page 161: ...anifold If you are unable to run the decontamination protocols due to a system failure perform the following alternate decontamination procedure to disinfect the internal tubing and manifolds Caution Be sure to check the percentage NaClO of the bleach you are using this information is printed on the side of the bottle Commercial bleach is typically 10 NaClO in this case prepare a 1 20 dilution Hou...

Page 162: ... visually or by performing qualification tests Decontaminate the Vacuum Filtration System First disinfect the tubing then remove and soak the carrier Decontaminate the tubing Run the predefined protocol QC_96 384_VAC30 10_TEST depending on your instrument model with disinfectant as the fluid supply to clean the tubing and hardware used to perform filter plate assays 1 Connect a supply bottle fille...

Page 163: ...d W DECONTAMINATE DECON_STEP1 STEP2 STEP3 W LONG_SHUTDOWN PURGE_SYSTEM_WITH_AIR n Two supply bottles are required for this procedure one for disinfectant and one for rinse Except Buffer Switching models require four bottles altogether two of each solution 1 Empty the waste bottle 2 Prepare an aqueous solution of 0 5 sodium hypochlorite NaClO or bleach 3 Fill one or two supply bottles with at least...

Page 164: ... Instrument After performing the Long Shutdown Prepare for Storage or Shipment protocols l Turn off the instrument and disconnect the power cord l Store it on a flat surface that is relatively free of vibration in a dust free and particle free environment l Protect the instrument from temperature extremes that can cause condensation within the unit and from corrosive fumes and vapors l Store the i...

Page 165: ...tly position the manifold s on the rails they only fit one way the side with the recessed channel openings faces the washer Make sure there are no gaps between the manifold and the washer Change the Instrument Settings When you change the type of manifold e g from a 96 tube to a 192 tube manifold you must tell the instrument which manifold is installed LHC Keypad 1 Select Tools Instrument Utilitie...

Page 166: ...ove the six screws around the perimeter of the top of the carrier 3 Lift off and set aside the top plate and remove the gasket o If the grate located under the gasket needs to be cleaned rinse it under tap water Allow the grate to dry before placing it and the gasket in the carrier 4 Place the new gasket in the carrier correctly positioning the notch with the corresponding indentation on the carri...

Page 167: ...cifications Qualification Overview 148 Qualification Schedule 149 System Self Test Verify Information 149 Sensor Test Fluid Waste Plate Detection 150 Ultrasonic Cleaner Test 151 Liquid Testing the 405 Microplate Washer LS 152 Washer Liquid Tests 154 Vacuum Filtration Evacuation Efficiency Test 165 Qualify the Verify Technology Sensor 167 ...

Page 168: ...se Precision This test measures the variability of volumes dispensed from tube to tube across the manifold l Buffer Switching This test measures the variability of volumes dispensed from tube to tube across the manifold when using buffer switching l Vacuum Filtration Evacuation Efficiency When applicable this test measures the total residual volume of the plate after evacuation to verify it is per...

Page 169: ...ists and Logbooks are included for recording results Contact your local dealer for more information Tests IQ OQ PQ Initially Annually Monthly Unpacking Installation and Setup ü Enable Test System Sensors ü ü System Self Test and Checksum Test ü ü ü Shake Test ü Ultrasonic Cleaner Test ü Washer Evacuation Efficiency Test ü ü Washer Dispense Precision Test ü ü Vacuum Filtration Evacuation Test ü ü B...

Page 170: ...f the instrument fix the problem and then turn the instrument back on and re run the test If the problem is not something you can fix contact BioTek s Technical Assistance Center Record Basecode Record the software part number and version installed on the 405 LS LHC Keypad 1 Select Tools Instrument Utilities 2 Select the Software tab and from the Instrument Software Information section record the ...

Page 171: ...y the expected error code 1737 no plate detected Ultrasonic Cleaner Test Perform this test to verify that the cleaner is operating properly 1 Fill a supply bottle with one liter of deionized water Buffer Switching models need two bottles connected to valves A and B 2 Select Quick Sonic from the main menu Set the Duration to 002 2 minutes 3 Run the program 4 While the AutoClean program is running t...

Page 172: ...tallation l Annual Buffer Switching Test Measures the variability of volumes dispensed from tube to tube across the manifold for each valve when the washer is equipped with buffer switching l Verify Sensor Test Compares the ultrasonic level sensor s measurement precision against a microplate absorbance reader s determination to ensure accurate repeatable volume level detection Perform the tests ap...

Page 173: ...ffer retry the tests using the recommended solutions Plate Reading l If you are using one of BioTek s keypad based readers such as the ELx800 or ELx808 ensure that the reader is not running in Rapid mode To check the setting select UTIL à READ and cycle through the options until READ IN RAPID MODE appears Set it to NO l The absorbance of blue dye solutions should be measured at 630 450 or 405 nm T...

Page 174: ...e lower the residual per well the better the evacuation efficiency of the washer A known solution is dispensed into all wells of a previously weighed microplate Aspiration is performed and the plate is re weighed The total residual fluid is calculated based on the weight difference and this value is divided by 96or 384 as appropriate to obtain the mean residual weight In addition a diagnostic tool...

Page 175: ...have changed the file location 1 Click the Open button and locate the 405 LS folder Choose the 1Buffer or 4Buffer folder to match your instrument s Buffer Switching capability 2 Open the Prime and Maintenance or QC Protocols folder 3 Select the required protocol BioTek recommends customizing these predefined protocols as described in the topic Customize the Predefined Protocols on page 41 Washer Q...

Page 176: ...30 450 nm l Liquid Test Worksheets at the end of this chapter for recordingdata and results l Deionized water l Test solutions For this manifold Sol 1 Sol 2 Sol 3 Sol 4 96 Tube 1000 mL 100 mL 1200 mL n a 192 Tube 2000 mL 100 mL n a 1440 mL n These volumes are sufficient for performing the dispense tests and standard and diagnostic Evacuation Efficiency tests In most cases enough fluid will be left...

Page 177: ...ative SOLUTION 3 Dispense Precision Solution for 96 Tube manifolds Mix 1180 mL of deionized or distilled water with 20 mL of Solution 2 SOLUTION 4 Dispense Precision Solution for 192 Tube manifolds Mix 1420 mL of Solution 1 with 20 mL of Solution 2 Dispense Precision Test 96 n This test is designed for 96 Tube manifolds Find the Dispense Precision Test 192 on page 161 n Find a list of the supplies...

Page 178: ...s Standard Deviation function e g Microsoft Excel s STDEV a Calculate the Standard Deviation b Calculate the sum of the OD values for all 96 wells then divide by 96 to determine the Mean OD for the plate c Calculate the CV Standard Deviation Mean OD 100 The CV should be 3 0 If the CV is greater than 3 0 one or more dispense tubes may need to be cleaned Run AutoClean if available and or remove the ...

Page 179: ...han 0 002 g or if one or more wells appear to have much more liquid than the others the washer failed the test Troubleshoot as follows If the test fails once o If the problem appears to be related to particular wells clean those aspiration tubes run AutoClean and or remove the manifold and thoroughly clean the tubes with the stylus See Remove and clean the washer manifold on page 131 When finished...

Page 180: ...nd its Residual Weight Each well s Residual Weight should be 0 002 g If one or more wells have a Residual Weight greater than 0 002 g review the data to determine which well or wells is causing the problem o If the problem appears to be related to particular wells clean the associated aspiration tubes run AutoClean and or remove the manifold and thoroughly clean the tubes with the stylus See Remov...

Page 181: ...1 68 g for the 96 Tube test l 30 72 grams 10 between 27 65 g and 33 79 g for 192 Tube test l If the weight falls above this range the valve may be defective Contact BioTek l If the weight falls below this range the valve may be contaminated with fungi or proteins and must be cleaned using an appropriate enzyme alcohol or a diluted bleach solution depending on the contaminant See Removing Protein R...

Page 182: ...ave a spreadsheet software program enter export all 384 values into a spreadsheet and apply your program s Standard Deviation function e g Microsoft Excel s STDEV a Calculate the Standard Deviation b Calculate the sum of the OD values for all 384 wells then divide by 384 to determine the Mean OD for the plate c Calculate the CV Standard Deviation Mean OD 100 The CV should be 4 0 If the CV is great...

Page 183: ...eight is greater than 0 002 g or if one or more wells appear to have much more liquid than the others the washer failed the test Troubleshoot as follows If the test fails once o If the problem appears to be related to particular wells clean those aspiration tubes run AutoClean and or remove the manifold and thoroughly clean the tubes with the stylus See Remove and clean the washer manifold on page...

Page 184: ...step 1 to find the Residual Factor l For each well divide its OD value by the Residual Factor to find its Residual Weight Each well s Residual Weight should be 0 002 g If one or more wells have a Residual Weight greater than 0 002 g review the data to determine which well or wells is causing the problem o If the problem appears to be related to particular wells clean the associated aspiration tube...

Page 185: ...s Plate Predefined Protocol Volume Vacuum Time 96 well QC 96_VAC30_TEST 300 µL well 30 seconds 384 well QC 384_VAC10_TEST 80 μL well 10 seconds 4 When the protocol is finished remove the plate and blot the bottom of the plate on a paper towel to remove any droplets 5 Weigh the plate immediately This is the Total Residual Weight in grams 6 Visually inspect the plate and note if any wells appear to ...

Page 186: ...free filter plate was used l The vacuum filtration plate carrier s gasket is clean and has not been damaged by previous use or mishandling Correct these conditions and rerun the test or contact BioTek TAC for assistance 405 Microplate Washer LS ...

Page 187: ...he washer and fully prime the system If you purchased the 405 LS IQ OQ PQ procedure l If you have Gen5 create a new experiment with the Gen5 Verify Technology protocol VerifyCal prt shipped with the IQOQPQ procedure Otherwise open the Excel spreadsheet provided on the 405 LS Operator s Manual CD in preparation for recording and calculating test results Run Verify Test Plate Alignment To make sure ...

Page 188: ... 7 Use the absorbance reader to read the plate at 630 nm and 450 nm Gen5 users select OK l If you do not have Gen5 copy and paste the Delta transformation reading results 630 nm 450 nm into the spreadsheet provided in the Supporting Data section paste data into the Dd dispense distance grid l If you have Gen5 the Power Export containing the reading results opens Fill in the other data fields for w...

Page 189: ...the correct orientation Was there a long delay between steps allowing fluid to evaporate l Inspect the plate for damage Replace the plate according to the prescribed procedure if the plate is cracked warped scratched or otherwise damaged l Inspect the washer components o Is the Verify sensor s collimator clean and properly installed Clean the Verify Level Sensor on page 133 o Have the plate carrie...

Page 190: ...170 Chapter 5 Qualification 405 Microplate Washer LS ...

Page 191: ...lculations Standard Deviation calculate using spreadsheet program Mean OD sum of all wells number of wells Coefficient of Variation Standard Deviation Mean OD x 100 CV 3 0 q Pass q Fail Date Test Performed By 405 LS Qualification Procedures 2012 BioTek Instruments Inc ...

Page 192: ...dual Weight 96 g Mean Residual Weight 0 002 g q Pass q Fail Evacuation Diagnostics Test check here q if not performed Mean OD for the plate Sum of all wells 96 Residual Factor Mean OD Mean Residual Weight Calculate the Residual Weight for each well well OD Residual Factor Every Residual Weight per well 0 002 g q Yes q No Date Test Performed By 405 LS Qualification Procedures 2012 BioTek Instrument...

Page 193: ...lls CV Standard Deviation Mean OD x 100 CV 3 0 q Pass q Fail Calculations for Valves A D Annual OQ only check here q if not performed Total Dispense Weight 28 8 g 10 25 92 g 31 68 g Valve A grams q Pass q Fail Valve B grams q Pass q Fail Valve C grams q Pass q Fail Valve D grams q Pass q Fail Date Test Performed By 405 LS Qualification Procedures 2012 BioTek Instruments Inc ...

Page 194: ...alculations Standard Deviation calculate using spreadsheet program Mean OD sum of all wells number of wells Coefficient of Variation Standard Deviation Mean OD x 100 CV 4 0 q Pass q Fail Date Test Performed By 405 LS Qualification Procedures 2012 BioTek Instruments Inc ...

Page 195: ...dual Weight 384 g Mean Residual Weight 0 002 g q Pass q Fail Evacuation Diagnostics Test check here q if not performed Mean OD for the plate sum of all wells 384 Residual Factor Mean OD Mean Residual Weight Calculate the Residual Weight for each well well OD Residual Factor Every Residual Weight per well 0 002 g q Yes q No Date Test Performed By 405 LS Qualification Procedures 2012 BioTek Instrume...

Page 196: ...lls CV Standard Deviation Mean OD x 100 CV 4 0 q Pass q Fail Calculations for Valves A D Annual OQ only check here q if not performed Total Dispense Weight 30 72 g 10 27 65 g 33 79 g Valve A grams q Pass q Fail Valve B grams q Pass q Fail Valve C grams q Pass q Fail Valve D grams q Pass q Fail Date Test Performed By 405 LS Qualification Procedures 2012 BioTek Instruments Inc ...

Page 197: ...ates Serial Number Test Results Verification that wells are consistent in appearance c Pass c Fail Residual Weight g Residual Weight 1 2 g c Pass c Fail Date Performed By If required Reviewed Approved By 405 LS Qualification Procedures 2012 BioTek Instruments Inc ...

Page 198: ...tes Serial Number Test Results Verification that wells are consistent in appearance c Pass c Fail Residual Weight grams Residual Weight 4 0 g c Pass c Fail Date Performed By If required Reviewed Approved By 405 LS Qualification Procedures 2012 BioTek Instruments Inc ...

Page 199: ...Chapter 7 Troubleshooting This chapter provides guidelines for error recovery and troubleshooting performance problems Troubleshooting 180 General Operation Troubleshooting 182 ...

Page 200: ...tentially common error especially when using the Predefined Protocols a serial write error is easily fixed by correcting the COM port setting defined in the protocol 810D To communicate instrument must be at main menu Home screen LHC Users Similarly the 810D message appears when the instrument is busy for example when AutoPrime is running The LHC can only talk to the instrument when its main menu ...

Page 201: ...Troubleshooting 181 disconnect the BioStack from the 405 LS and change the Instrument Setting for the BioStack Press Setup Menu BIOSTK CONF MANUAL BioTek Instruments Inc ...

Page 202: ...isaligned carrier or manifold Contact BioTek TAC Incorrect manifold setting Make sure the manifold setting matches the installed manifold LHC Tools Instrument Utilities Washer Touch screen Instrument Options Microplate Scratches Problem Possible Cause What To Do Scratches on microplate bottom Microplate dispense or aspiration height adjustment too low Change the Dispense or Aspirate height the Z a...

Page 203: ...se PN 46055 Replace the Vacuum Pump Fuse on page 145 Repeated blown fuses Vacuum Dissipate Delay is set too low for the volume of the waste bottle See above If not enough time is allowed for the vacuum to dissipate the pump will try to start while it is under a vacuum The pump draws excessive current and blows the fuse Pump has been flooded Remove the head from the pump and inspect it for corrosio...

Page 204: ...timize protocols to improve evacuation on page 40 Aspiration tubes not properly positioned horizontally in wells If none of the tubes are bent try adjusting the horizontal aspirate position X Y axis in the protocol Microplate not level in carrier or strips not level in holder Reseat microplate in carrier or strips in holder Make sure the carrier is clean Try a different microplate or strip holder ...

Page 205: ...inse fluid Fill bottles with appropriate fluid Ensure bottles are clean and do not contain particles or organic material Unable to dispense fluid System not primed Large air pockets in tubing Run W DAY_RINSE Insufficient suction clogged tubing or faulty valve Perform Washer Maintenance If the problem persists contact BioTek TAC Plate overfills floods Dispense height too high The aspirate tubes are...

Page 206: ...e is no vacuum contact BioTek TAC Uneven dispensing of fluid wells not filled Clogged dispense tubes on the washer manifold Remove and clean the washer manifold on page 131 Manifold or tubing not adequately primed Run W DAY_RINSE once or twice Dispense flow rate too low Flow rate 1 or 2 CW is used with 384 well plates Define a higher dispense Flow Rate Microplate aspiration height adjustment too h...

Page 207: ...tering wells correctly Microplate not properly seated or strips not level Reseat microplate in carrier or strips in holder Make sure the carrier is clean Try a different microplate or strip holder If the problem is unresolved the carrier may have to be realigned Contact BioTek TAC Aspirate tubes position is too wide for a movement Change the horizontal X or Y axis aspirate position in the protocol...

Page 208: ...sher manifold setting matches the installed manifold 96 192 tube LHC Tools Instrument Utilities Washer Manifold Selection Keypad Setup Menu g MANIF Communication Errors Here are some guidelines for troubleshooting communication errors between the 405 LS and the computer 6045 A potentially common error especially when using the Predefined Protocols a serial write error is easily fixed by correcting...

Page 209: ... will be displayed when a test fails 2 Make sure the USB cable is in perfect condition and properly attached to the port defined in the Instrument Settings dialog e g COM 1 Review the LHC Help Topic Select Help gt Help Topics and search for About COM Ports About COM Ports to learn about virtual COM ports when using a USB cable Correct and reboot both PC and instrument Test communication 3 Confirm ...

Page 210: ...190 Chapter 7 Troubleshooting 405 Microplate Washer LS ...

Page 211: ...Appendix A Error Codes A listing of potential error codes and possible solutions for resolving them System Error Codes 192 405 LS LHC Software Error Codes 200 ...

Page 212: ...e plate carrier rails and glide strips using mild detergent and hot water 70 isopropyl alcohol or ethanol Restart the instrument If the error occurs again contact BioTek TAC 211 221 Carrier Y motor didn t find home opto sensor transition Carrier Y motor didn t find autocal jig opto sensor transition Run self test If error reoccurs contact BioTek TAC 212 222 Dispense head motor didn t find home opt...

Page 213: ...reoccurs contact BioTek TAC 402 Dispense head motor failed positional verify Run self test If error reoccurs contact BioTek TAC 403 Wash head motor failed positional verify Service Only Contact BioTek TAC 600 606 Specified motor currently in use Service Only Contact BioTek TAC 700 70F Invalid motor number specified Service Only Contact BioTek TAC 900 Calibrationon failed The measured or calculated...

Page 214: ...detected Contact BioTek TAC 1010 Checksum error downloading basecode Contact BioTek TAC 1250 Internal RAM test error on the UI processor Contact BioTek TAC 1251 Internal RAM test error on the MC processor Contact BioTek TAC 1260 Stack test error on the UI Contact BioTek TAC 1261 Stack test error on the MC Contact BioTek TAC 1400 No vacuum pressure detected after turning on the vacuum pump Make sur...

Page 215: ...e Clear or other height settings Review the protocol parameters and instrument settings to identify a correction 1407 Invalid step type The protocol may have been created for a different instrument it is not compatible with this instrument 1408 Invalid plate geometry Contact BioTek TAC 1409 Invalid plate carrier type Contact BioTek TAC 1410 Incompatible hardware Vacuum filtration requested but eit...

Page 216: ...g through the manifold during an operation Flow detection errors Make sure the supply bottle is full and properly connected the tubing is not kinked blocked etc and the correct port valve is selected 1505 No buffer detected at the end of a wash protocol 1506 The requested carrier Y axis position is out of range Contact BioTek TAC 1507 Internal valve transition error Contact BioTek TAC 1508 Pre dis...

Page 217: ...nable to read vac filtration sensor This is expected if you are running with very low pressure in this case disable the sensor Otherwise check for leaks e g bottle cap 1600 160C The onboard storage space allocated for this function has been used up Use the LHC Manage Memory control to reallocate space 160D Not a valid step Contact BioTek TAC 1700 1709 Q model level sensor errors Contact BioTek TAC...

Page 218: ...anceled by user Restart instrument if this message is unexpected 8100 Communications NAK Contact BioTek TAC 8101 Timeout while waiting for serial message data Contact BioTek TAC 8102 Instrument busy and unable to process message Contact BioTek TAC 8103 Receive buffer overflow error Contact BioTek TAC 8104 Checksum error Contact BioTek TAC 8105 Invalid structure type in byMsgStructure header field ...

Page 219: ...810F The command was received while the software was not ready to accept that command Contact BioTek TAC A00 Invalid plate type requested Service Only Contact BioTek TAC A100 A10F Software device not available Service Only Contact BioTek TAC A200 Version strings for multiple microprocessors do not match Service Only Contact BioTek TAC A301 5v logic power supply level error Service Only Contact Bio...

Page 220: ...escription Help 6000 General communication error during download See Communication Errors on page 188 6001 COM port created by USB converter no longer active See Communication Port 6002 Invalid basecode part number instrument is not an 405 LS Service Only Contact BioTek TAC 6003 Invalid Basecode Data Version basecode needs to be updated Contact BioTek to obtain latest basecode 6004 No rows are sel...

Page 221: ...pre dispenses Service Only Contact BioTek TAC These codes indicate an unexpected software error that cannot be fixed without BioTek support 6019 Invalid horizontal dispense position 6020 Invalid dispense height 6021 Invalid plate clear height 6022 Invalid column selection value must be 0 or 1 6023 Invalid protocol step type 6024 The Definition String contains invalid data 6025 Manifold conflict be...

Page 222: ...he Predefined Protocols to avoid this error in future When controlling the BioStack with the LHC make sure the instrument s BioStack setting is Manual 6045 Serial write error 6046 Serial read error 6047 Checksum error Contact BioTek TAC 6048 Serial NAK error Make sure the COM port setting is correct and the cable is properly connected Restart the instrument If error reoccurs contact BioTek TAC 604...

Page 223: ...lowed with this protocol step Modify the protocol to change the plate type 6065 Too few data bytes received from the instrument Contact BioTek TAC 6066 Ultrasonic cleaning assembly is not installed The protocol may have been created for a different instrument Make sure the instrument settings match the current instrument and modify the protocol 6074 Invalid Y axis offset value Service Only Contact...

Page 224: ...tensity selected 6090 Invalid Washer buffer selected 6091 Invalid Washer Aspirate Delay value 6092 Invalid Washer Aspirate Travel Rate value 6093 Invalid Wash Cycles value 6094 Invalid Wash format selected 6095 Invalid Wash Sectors selected 6096 Wash Aspirate Delay value is required 6100 This functionality requires the software to be registered You must register the software with BioTek Select Hel...

Page 225: ...autions sont spécifiques à chaque appareil Le cas échéant l information est précédée par la catégorie d instruments par exemple Détection Liquid Handling ou modèle par exemple EL406 BioStack S il n y a pas une telle indication l information s applique à tous les produits Warnings Avertissements Operate the instrument on a level stable surface away from excessive humidity Faites fonctionner l appar...

Page 226: ...spécifiée pour le système doit être impérativement utilisée L utilisation d une prise de courant incompatible peut produire un choc électrique et un incendie Warning Electrical Grounding Never use a plug adapter to connect primary power to the external power supply or the instrument Use of an adapter disconnects the utility ground creating a severe shock hazard Always connect the power cord direct...

Page 227: ...exposés à des fluides Warning Unspecified Use Failure to operate the equipment according to the guidelines and safeguards specified in this manual could result in a hazardous condition Attention Utilisation non spécifié Si l appareil n est pas utilisé selon les lignes directrices et les garanties spécifiées dans ce manuel vous risquez d entrainer une situation dangereuse Warning Software Quality C...

Page 228: ...xported via computer control must be thoroughly analyzed by the operator Détection Attention Protocole pour lecteur de réduction des données Pour les lecteurs avec claviers Le logiciel d analyse inclus dans ces lecteurs signalera lorsque les touches contrôle sont hors de portée Le logiciel signalera sur les données avec les drapeaux d erreur appropriés afin que l utilisateur puisse déterminer le c...

Page 229: ...ing instrument operation the pipette shuttle may be moving There are potential pinch points on the mechanism and opportunities for skin puncture with the pipette tips Keep hands completely out of the way of the pipette shuttle when the instrument is in operation Attention Pièces en mouvement Le symbole sur la navette pipette indique un risque de blessure À tout moment de l utilisation la navette p...

Page 230: ...our un bon fonctionnement les températures ambiantes doivent rester dans les limites indiquées dans la section Caractéristiques techniques du manuel de l opérateur La performance peut être affectée si la température fluctue au dessus ou en dessous de cette fourchette La limite de température de stockage est plus flexible Caution Sodium Hypochlorite Do not expose any part of the instrument to the r...

Page 231: ...s of the microplate carrier movement rail springs and other hardware Attention Solution tampon Bien que de nombreuses précautions ont été prises pour s assurer que l instrument est aussi résistant à la corrosion que possible l instrument n est pas étanche et les liquides peuvent s infiltrer dans les composants sensibles Assurez vous que toute solution tampon déversée est essuyée Une exposition pro...

Page 232: ...the barcode scanner Attention Miroir lecteur de code barres Ne pas rayer ou endommager le miroir lors du déballage ou lors de l installation du lecteur de code barres BioStack Caution Aligning Posts When installing the BioStack s four aligning posts use caution not to cross thread these parts Finger tighten only See the installation instructions Attention Messages pour alignements Pour l installat...

Page 233: ...c Advantage 125 Bottles Clean filter 130 Connection 18 Bottom Wash 70 BSA 124 125 Buffer Switching 57 installation 22 QC test 160 C Carrier setting 107 Cell Wash 68 84 aspirate rate 68 Strategy to preserve cells 85 Changing the wash manifold 107 Cleaning 125 128 132 140 Removing proteins 124 Columns Dispensing to 50 COM Port 26 42 55 57 CommonAppDataFolder 56 Communication Errors 188 Copy a protoc...

Page 234: ...e carrier setting 107 Change the plate clearance setting 108 Change the washer manifold set ting 107 enter a negative value 52 Name a protocol 49 Shake the plate 51 Humidity limits 9 I Incubate a plate at room tem perature 51 66 Instrument Settings 56 57 Defining 26 Instrument Utilities 122 Adjust Utility 108 AutoPrime 122 Verify sensor 72 Interface Software 56 Intermediate Waste System Installing...

Page 235: ...te Type 111 Defining 47 50 supported 9 Port link 42 55 57 Power Supply 9 Power users 56 Pre Dispense 67 Prevent fluid loss during wash 87 Prime tips 67 Priming trough how to empty 46 Processing patterns 111 Protein Preventing clogging 124 Protocol Copying 52 Deleting 52 Input a negative number 52 Naming 49 Shake and Soak 51 Protocols Customize 41 56 Predefine 56 Q QC 56 QC testing 152 Qualificatio...

Page 236: ...183 turn off 46 Vacuum Dissipate 27 Vacuum Filtration 129 133 QC test 165 Vacuum Filtration Carrier decontaminate 142 replace gasket 146 Vacuum on based on volume 87 Valve module installation 22 Verification instrument testing 152 Verify Technology 3 72 maintenance 133 qualify 167 replace the test plate 134 W Wash about 38 extra turbulence 70 Gentle cell wash 84 Magnetic beads 89 Polystyrene beads...

Page 237: ...Windows Common Applications Data Folder 209 Folder Windows 7 56 Windows Vista 56 Windows XP 56 Worksheet Test results 165 BioTek Instruments Inc ...

Page 238: ......

Page 239: ...phragm 9 Neoprene Manifold channel end seals 10 PPO polyphenylene Oxide Noryl Vacuum switch internal disc 11 Polycarbonate Vacuum switch body Vacuum filtration intermediate waste bottle direct drain intermediate waste bottle 12 Polyethylene Buffer bottle 13 Polypropylene Outlet fitting fittings in bottles inline fittings float ball bottle caps vacuum filtration module bulkhead fittings 14 Polystyr...

Page 240: ...B C D A A ND B A A D ND A Hydrogen Peroxide 10 B B D A A A A A B A A A A A A C ND A A A Isopropyl Alcohol 70 B A A A B D A A B A A B A A B A ND A A A Methyl Alcohol 70 A A A A A B A A A A B A A ND A A B A A A Methylene Chloride B B B C D C A ND D D D D B D D A D D D B Phosphoric Acid 40 D D D C A B A A B A A A A B B A A C A A Propylene Glycol B B B B C A A ND C ND B A A A C A ND A ND A Sodium Chlo...

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