Biotek 405 LS, Operator'S Manual

Page: 110 / 240

90 | Chapter 3: Operation
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Flat magnet: place in the plate carrier so the text on the side of the magnet is readable;
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Ring magnet: place in the plate carrier with the small round magnets visible, facing
upwards.
Realign the BioStack with the Magnet Installed
Using the magnet increases the effective height of the carrier surface (generally by
at least 1 mm). This shift in the plate position requires a comparable adjustment to
the BioStack’s gripper movement. Realign the BioStack before using it with the
magnetic bead assays.
Refer to the BioStack Operator’s Manual for detailed instructions of the alignment
procedure. To help get you started:
1. Place the magnet in the carrier and a microplate on top of it.
2. Launch the
BioStack Alignment Utility
:
LHC Keypad
Tools> BioStack Utilities> Alignment Utility SETUP >BIOSTK > ALIGN
3. HOME the BioStack and Begin Realignment.
4. Lower the claw until a 0.050” (1.3 mm) gap between the bottom of the plate and
the top of the gripper fingers is achieved and save the gripper position.
5. Put the microplate in the input stack and Verify the alignment.
Remember to realign the BioStack for non-bead assays, when applicable.
Perform Magnetic Bead Assays
For the best results when performing biomagnetic separation assays:
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Use the Manifold Stop Screw Adjustment Kit on page 94, if necessary.
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Realign the BioStack with the Magnet Installed
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Change the Magnet Height Offset on page 93
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Optimize Magnetic Bead Protocols below
Optimize Magnetic Bead Protocols
Here are some suggestions to consider for optimizing your magnetic bead assays:
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Plate Type : Flat-bottom plates are recommended for magnetic bead assays because
more of their well surface sits closer to the magnet, resulting in increased magnet
strength, than with other plate formats. If you must use round-bottom plates, increase
405™ Microplate Washer LS