Molecular Devices FLIPR Tetra, User Manual

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FLIPR
®
Tetra High Throughput Cellular Screening System User Guide
0112-0109 H 177
Precipitation in the Reagent Buffer
The FLIPR
®
Calcium Assay Kit is compatible with numerous buffers. Use
buffers shown to work in previously established assays, if available.
Response is Smaller than Expected
The agonist and antagonist may stick to the tips and trays. Use up to
1% BSA in all compound buffer diluents and presoak tips in compound
buffer with up to 1% BSA.
Apparent Well-to-Well Variation is Observed
AquaMax DW4 or equivalent dispenser is recommended for use with
additions of cells or dye prior to the assay on the FLIPR
®
Tetra System if
apparent well-to-well variation is observed. In some cases allowing the
plates to stand at room temperature prior to use or adding a single mix
cycle in the compound or assay plate may decrease well-to-well
variation.
FLIPR Membrane Potential Assay Kit Protocol
This section provides the following information and procedures:
• Materials required for the assay
• Cell preparation guidelines
• Dye loading procedure
• Troubleshooting tips for membrane potential assays
Required Materials
The following materials are required for running a membrane potential
assay:
Note: Do not use the same source plate for presoaking and compound
addition when using a 384 Pipettor head in the FLIPR
®
Tetra System.
Instead, use a 'Boat' for the presoak.
Item Source
FLIPR ® Tetra System with Membrane Potential
Optics Kit installed:
FLIPR
®
Tetra System LED Module 510–545 nm
FLIPR
®
Tetra System Emission Filter 565–625
nm
Molecular Devices
See Appendix C