Molecular Devices FLIPR Tetra, User Manual

Page: 59 / 302

FLIPR
®
Tetra High Throughput Cellular Screening System User Guide
0112-0109 H 59
SynchroMax™ Automation
The SynchroMax Automation command is only enabled if you have a
SynchroMax ET installed.
When you open the
SynchroMax dialog from Instrument >
SynchroMax Automation , ScreenWorks Software automatically goes
into Remote Mode , giving control of the instrument to the
SynchroMax ET software. ScreenWorks Software returns to Manual
Mode when the SynchroMax dialog closes.
To run a series of experiments with the SynchroMax ET, first open the
protocol file that you want to use in ScreenWorks Software. Only one
protocol is used in a run, as many times as the SynchroMax ET supplies
plates for it.
Having prepared the protocol, open the
SynchroMax dialog and select
a Stack Layout Template . These files specify, for each plate location
in the five-position stage, how many plates will be loaded to that
position, from which stack, and where they will be delivered to after
use. A number of Stack Layout Templates are supplied—select the
one that suits your assay.
When a
Stack Layout Template is opened in the dialog the graphic in
the dialog displays which stacks plates should be loaded in. You can use
this as a guide for loading the plates. All plates in a single stack should
be of the same type.
If there are any stage positions that will use the same plate throughout
the duration of the run, these should be loaded manually before the run
begins. Stage positions that will have plates brought to them during the
run should be empty at the start of the run.
Save When clicked, saves the signal test as an ASCII text file
(*.sig).
Print When clicked, prints the signal test.
Image Viewer Displays the CCD image showing where RLU values were
derived for the signal test.
Show > Hide Mask —Shows or hides the mask used in
the test, indicating the pixel area used to derive the RLU
value for each well.
View Image —Refreshes signal test image.
Item Description