Molecular Devices FLIPR Tetra, User Manual

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FLIPR
®
Tetra High Throughput Cellular Screening System User Guide
0112-0109 H 293
FLIPR Membrane Potential Assay Kit Consumables
Equipment and Supplies Suggested to Perform
Assays with Your FLIPR Tetra System
• AquaMax
®
DW4 96/384/1536 Dispenser/Washer, Molecular
Devices.
• 5 mL, 10 mL, 25 mL sterile serological pipettes.
• Rechargeable pipettor for 2–25 mL pipettes.
• Sterile tissue culture water.
• Gloves.
• Culture medium to grow cells.
• EDTA and Trypsin/EDTA to lift cells.
• Hemacytometer and counter.
• Sterile test tubes 15 mL and 50 mL or smaller tubes for
compounds dilutions.
• 1 N NaOH solution to dissolve probenecid.
Table C-15
Item
Suggested
Supplier
Phone Number Item #
FLIPR
®
Membrane
Potential Assay Kit
Molecular D1-800-635-5577
+1-408-747-1700
R8034 (BLUE)
R8123 (RED)
FLIPR
®
Tetra System
Membrane Potential Kit:
FLIPR
®
Tetra
System LED
Module, 470–495
nm
FLIPR
®
Tetra
System Emission
Filter, 515–575 nm
Molecular D1-800-635-5577
+1-408-747-1700
0200-6207
Hank’s Balanced Salt
Solution (10X stock)
Gibco +1-800-828-6686 14065-056
HEPES buffer solution
1X
Irvine Scientific +1-800-437-5706 9319
Carbachol
(receptor-mediated
positive control)
Sigma +1-800-325-3010 C4382