FLIPR
®
Tetra High Throughput Cellular Screening System User Guide
0112-0109 H
71
Processes can be added to the end of a protocol, or, using the right
edge of a new process you drag over, inserted between other processes
already in the protocol, providing you do not attempt to insert between
processes that are internally linked, for example
,
a
Transfer Fluid
and
associated
Mix with TF
.
As each new process is dragged to the Experiment window, a new page
with configuration options for the process is added to the file. The new
page is automatically displayed.
Similarly, as you click on the processes above, the sheet associated
with that process, containing its configuration settings, comes forward.
Deleting Processes from the Protocol
To delete processes that are not connected click on the process icon in
the Experiment window and click the
Delete
key.
To delete a connected process—
Mix with TF
or
Read with TF
—go to
the
Transfer Fluid
process that it is associated with and uncheck the
option for the process.
Process Icon Colors
The
Settings
and
Analysis
processes’ icons are green to indicate that
they are required to run an experiment. These processes’ are always
present and cannot be deleted.
The
Transfer Fluid
,
Mix Fluid
,
Wash Tips
or
Pins
,
Wash Reservoir
,
Blot Pins
,
Pause Pipettor
and
Finish with Source
processes icons
are blue to indicate that they are
liquid
handling steps. These steps are
always in series with each other; no two of them will ever occur
simultaneously.
The
Read
process icon is orange to indicate that it will occur in parallel
with any liquid handling process, if possible.
However, a read can be
added through the
Transfer Fluid
process to indicate the liquid
handling will occur at a defined time in the experiment. These linked
processes will not begin until all read and liquid handling steps prior to
the
Transfer Fluid
linked with
Read with TF
are complete.
When
Mix with TF
or
Read with TF
processes are created (from the
options in a
Transfer Fluid
step), the icons of all the connected
processes change to purple, to show the linkage.
Settings Process
Settings is the first process in all protocols. Use it to define read modes,
plate positions, and data directories. You can also configure automatic
printing of selected results, automatic file naming, and set plate
temperature.
Select the green
Settings
icon in the Experiment window to view the
Settings
page where the settings are made.
Summary of Contents for FLIPR Tetra
Page 1: ...FLIPR Tetra High Throughput Cellular Screening System User Guide 0112 0109 H December 2011...
Page 12: ...Contents 12 0112 0109 H...
Page 16: ...System Overview 16 0112 0109 H...
Page 40: ...System Hardware Features 40 0112 0109 H...
Page 148: ...Exchanging Hardware 148 0112 0109 H...
Page 156: ...Calibration and Signal Test 156 0112 0109 H...
Page 196: ...Running an Experiment 196 0112 0109 H...
Page 232: ...Robotic Integration 232 0112 0109 H The following drawings illustrate these requirements...
Page 282: ...Data Processing Algorithms 282 0112 0109 H...
Page 294: ...Consumables and Accessories 294 0112 0109 H...
Page 298: ...Using AquaMax Sterilant 298 0112 0109 H...
Page 302: ...Electromagnetic Compatibility EMC 302 0112 0109 H...