Molecular Devices FLIPR Tetra, User Manual

Page: 160 / 302

Running an Experiment
160 0112-0109 H
components are installed, see the appropriate section in Exchanging
Hardware on page 129 for installation instructions.
Checking the System
Once a day, before running your first assay plate, run a Yellow Plate
Signal Test to ensure the system is performing according to
specifications. A yellow signal test plate is available for each
corresponding pipettor head supplied with the system.
CAUTION! Store the yellow signal test plate in a safe place away from
bright light on an even surface. Avoid scratching the plate bottom as
scratches can affect the standard deviation. A signal test plate does not
need to be run more than once a day unless system components such
as pipettor heads or LEDs are changed.
Running the Yellow Plate Signal Test
To run a Yellow Plate Signal Test:
1. Select Yellow Plate Signal Test from the Instrument >
Manual Operation menu or click the Yellow Plate signal test
button.
2. Set the following parameters:
3. Select the correct plate from the Select Plate list.
4. Select appropriate Read Mode .
5. Select the appropriate Excitation/Emission Wavelengths .
6. Set the Excitation Intensity to 50.
7. Set the Exposure Time to 0.1 seconds.
Note: Please do not attempt to change pipettor heads, wash
reservoirs, LED banks and filters while the instrument is running. All
features must be exchanged in the appropriate instrument mode.
Optics calibration will need to be performed when the instrument is
started for the first time after re-configuration of LEDs or emission
filters.
Note: Make sure that the system is turned on and the CCD camera is
cooled to -60 °C ± 2 °C when the EMCCD camera is present or -
20 °C ± 5 °C when the ICCD camera is installed to ensure proper
system functioning.