Molecular Devices FLIPR Tetra, User Manual

Page: 178 / 302

Running an Experiment
178 0112-0109 H
FLIPR Membrane Potential Assay Kit
The FLIPR
®
Membrane Potential Assay Kits from Molecular Devices
provide a fast, simple and reliable fluorescence-based assay for
detecting changes in voltage across the cell membrane. These kits are
designed to work in association with many receptors and ion channels
as well as both adherent and non-adherent cell lines. The assay is a
mix-and-read procedure in which cells are incubated with the kit
reagents for 30 minutes after which the signal is detected using a
FLIPR
®
System.
There are no intermediate wash steps involved and, typically, the assay
is complete one minute after addition of the agonist.
One of the following Membrane Potential Assay
Kits:
Evaluation Kit
MP Blue, explorer
MP Blue, bulk
MP Red, explorer
MP Red, bulk
Molecular Devices
R8128
R8042
R8034
R8126
R8123
Clear, flat-bottom, black- or clear-wall 1536-,
384- or 96-well plates
See Appendix C
Clear polypropylene source plates See Appendix C
FLIPR
®
Sytem
pipette tips, 96, 384 or 1536
gasket
See Appendix C
Cells in suspension —
Test compounds Specific to receptor
Growth medium Major Laboratory Supplier
(MLS)
Incubator MLS
Centrifuge MLS
Pipettor and sterile tips suitable for use with
microplates
MLS
1 N NaOH MLS
Item Source
Note: The Kit includes masking dye technology covered by issued and
pending patents including US 6,420,183, US 7,063,952 and EP
0,906,572, licensed exclusively from Bayer AG to Molecular Devices.