Molecular Devices FLIPR Tetra, User Manual

Page: 114 / 302

ScreenWorks Software Overview
114 0112-0109 H
Edit Dispense
(continued)
Speed —Enter speed at which to dispense (in μ L/s). Ideal
dispense speed takes into account the volume added, how
fast the signal increases in response to compounds and
strength of the attached cells at the bottom of the plate.
Expel Volume —Enter an additional volume (in μ L) the
pipettor dispenses over the dispense volume. Typically, this
value is equal to the aspirate Hold Volume value.
In a multiple dispense, only the last dispense can have an
Expel Volume .
If Mix Fluid After Dispense is selected, Expel Volume
automatically changes to zero for the dispense associated
with the fluid transfer, to ensure that tips remain in the well
prior to the mix step. However, the expel volume designated
in the dispense is transferred to the Mix with TF step, to
help ensure liquid is properly expelled from the tips after
mixing.
Removal Speed —Enter rate (in mm/s) at which tips are
pulled from well immediately after the dispense. This value is
adjusted for fluid viscosity, fluid volume in the well or if the
transfer is performed to a dry well.
Pause in Well —Enter time (in seconds) to pause with the
tips in the wells after dispensing and mixing (if specified)
before the next pipettor move.
Read intervals may occur simultaneously with this step.
Typically, the pipettor is paused during a read to prevent
disturbances in the imaging process.
Mix Fluid After
Dispense
Check this box to link a Mix Fluid process to the Transfer
Fluid process.
This automatically adds a Mix with TF step to the protocol,
and opens the configuration page for that step.
See Mix Fluid Process on page 117 for more information
about mixing options.
Note: This option is only available when Fluid
Transfer Type has a single dispense.
Item Description