FLIPR
®
Tetra High Throughput Cellular Screening System User Guide
0112-0109 H
37
Figure 2-9
LED configuration.
Emission Filters
A three-position filter slider holding up to three 60 mm diameter
interference filters is located in front of the CCD camera. The slider can
be alternated in front of the camera to separate out the emission band
of the dye being used. These filters can be used with a single excitation
wavelength or can be paired up with additional emission filters and
LEDs in a ratiometric experiment, for example
,
as excitation LEDs
alternate between two different wavelengths, filters change at the
same time so that each image taken by the camera matches the right
emission filter with the excitation LED bank. The most common FLIPR
®
Tetra System
configuration is a LED excitation wavelength of 470–495
nm with a 515–575 nm bandpass emission filter. For luminescence
experiments it is also possible to run without the filter.
Emission filters are user-changeable in approximately 5 minutes; see
for instructions. Once installed, filters are
mechanically sensed and the filter configuration can be viewed in the
Instrument Configuration
panel in ScreenWorks Software. The
instrument will prompt you to calibrate a new emission filter with
respect to an LED module if they are intended to be used in an
experiment.
Summary of Contents for FLIPR Tetra
Page 1: ...FLIPR Tetra High Throughput Cellular Screening System User Guide 0112 0109 H December 2011...
Page 12: ...Contents 12 0112 0109 H...
Page 16: ...System Overview 16 0112 0109 H...
Page 40: ...System Hardware Features 40 0112 0109 H...
Page 148: ...Exchanging Hardware 148 0112 0109 H...
Page 156: ...Calibration and Signal Test 156 0112 0109 H...
Page 196: ...Running an Experiment 196 0112 0109 H...
Page 232: ...Robotic Integration 232 0112 0109 H The following drawings illustrate these requirements...
Page 282: ...Data Processing Algorithms 282 0112 0109 H...
Page 294: ...Consumables and Accessories 294 0112 0109 H...
Page 298: ...Using AquaMax Sterilant 298 0112 0109 H...
Page 302: ...Electromagnetic Compatibility EMC 302 0112 0109 H...