Dionex UltiMate 3000 Series, Руководство по эксплуатации

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UltiMate 3000 Series:
SD, RS, and BM Pumps
Operating Instructions Page 117
Problem Probable Cause Remedial Action
Peak Broadening,
increased dead time The inner diameter of the
capillary to the detector is too
large. Change the capillary.
The filter frits on the solvent
lines are clogged. Check the filter for
permeability. Replace the
filter frit if necessary
(

page 48).
The capillaries are clogged. Replace the capillaries.
The sample loop is clogged. Replace the sample loop
(

Autosampler Manual ).
The proportioning valve is
defective. Contact Dionex Service.
The column is overloaded or
contaminated. Clean or replace the column.
The solvent is degraded. Use fresh solvent.
Additional peaks appear in
the injection peak. With gradients, the equilibration
time after the flush cycle is too
short or the dead volume is too
high. Increase the equilibration
time or eliminate possibly
existing dead volume.
Poor reproducibility The autosampler draws air from
the vial. There is not enough amount
of sample in the vial, the
needle height setting is
incorrect (

Autosampler
Manual ), or there are too
many replicates.
There are air bubbles in the
syringe. Flush the syringe
(

Autosampler Manual ).
There are air bubbles in the flow
path. Perform a wash cycle
(

Autosampler Manual ).
Non-degassed wash liquid is
used. Degas the wash liquid
(

Autosampler Manual ).
The draw speed for the sample is
too high. Reduce the draw speed.
The gas content of the sample is
too high or saturated. Reduce the draw speed.
Degas the sample if possible.
Carry-over occurs in the system. Flush the needle using an
appropriate solvent.
The needle is clogged or the
needle tip is deformed. Replace the needle
(

Autosampler Manual ).