Dionex UltiMate 3000 Series, Руководство по эксплуатации

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UltiMate 3000 Series:
SD, RS, and BM Pumps
Page 4 Operating Instructions

Do not place any heavy objects on the open front panel door. This may damage the door.

Always set a lower pressure limit for the HPLC pump. This prevents damage resulting
from leakage or from running the pump dry over a longer period. Activate liquid level
monitoring for the solvent reservoirs (

page 94).

To avoid that the pressure calibration of the pump is impaired, turn on the pump only
when the pump pressure is down. To ensure that the pressure is down, open the purge
valve before turning on the pump. If you are operating an ISO-3100BM, observe the
precautions on page 90.

Never run the pump dry. Damage to the pistons or the piston seals could result.

Before you start operating the pump, check the liquid level in the wash liquid reservoir
and refill the reservoir as needed. After turning on the pump, wait until the wash liquid
has passed all pump heads.

Always use fresh rear seal wash liquid.

Dionex advises against recycling the solvents. This may impair the performance of the
seals.

When connecting the capillaries, make sure that the connectors are free from
contaminants. Even minute particles may cause damage to the system, for example, to the
column.

After operation, rinse out buffers and solutions that form peroxides.

Before switching from buffer to organic solution, rinse the pump thoroughly with
deionized water.

When switching to another solvent, ensure that the new solvent is miscible with the one
contained in the pump. Otherwise, the pump can be damaged; for example, by
flocculation.

If the pump flow is interrupted for longer periods (> 1 hour), turn off the lamps in any UV
or RF detector connected to the pump. This will prevent evaporation in the flow cell.
If you want to connect an ESA Corona or Coulochem III detector to the pump, refer to
page 43 for details.

Always use the frits recommended by Dionex to prevent particulate matters from entering
the HPLC system. Using other frits may considerably affect the system performance.

Do not use stainless steel frits with the BM pumps; this renders the biocompatibility void.
Frits are used on the drawing side (in the solvent reservoirs) and on the high-pressure side
(in the inline filter).

If the mobile phase includes volatile or flammable solvents, avoid open flames and
sparks.