Dionex UltiMate 3000 Series, Руководство по эксплуатации

Страница: 98 / 236

UltiMate 3000 Series:
SD, RS, and BM Pumps
Page 84 Operating Instructions
5.5.6.2 Choosing the Seal-Washing Liquid
Observe the precautions for the composition of the seal washing liquid:

Make sure that the liquid used for rear seal washing is miscible with the solvent. This is to
avoid impairing the tightness of the pump.

Make sure that the seal washing liquid is compatible with the silicone tubing.

For reliable detector performance, make sure that the seal washing liquid is slightly
conductive. Standard HPLC-grade water with, for example, 5-10% isopropanol or
methanol, is appropriate.
NP applications
For these applications, isopropanol with 0.1% sulfuric acid (to ensure that the liquid is
conductive) is recommended. In addition, replace the silicone tubing and the detector of
the rear seal wash system with the Pharmed® tubing and the NP detector from the
Normal Phase (NP) kit for SD pumps (part no. 6040.1972).

If a liquid other than HPLC-grade water has to be used due to the miscibility of the
delivered solvent, make the liquid slightly conductive by using the appropriate additives.
(Do not use additives with a high salt content or additives that cause solid residuals upon
evaporation.) Be sure that liquid drops are present in the detector.
5.5.6.3 What happens ….
Correct functioning of the rear seal wash system
During the delivery period of the peristaltic pump, liquid reaches the detector of the rear seal
wash system. This means that the seal wash system performs correctly. The tubing is all right
and the peristaltic pump works correctly.
Malfunctioning of the rear seal wash system
If no drops reach the detector after maximum five minutes although the peristaltic pump is
pumping, this may indicate that the

Liquid reservoir of the rear seal wash system is empty.

Peristaltic tubing is blocked or crimped.

The seal wash tubing (silicone tubing) is pinched.

Detector of the system is dirty.
In all cases, the following message appears in the Chromeleon Audit Trail: "Rear seal wash
system has run out of wash solution". If the rear seal liquid cannot flow out properly to the
waste, the following message may appear: "The rear seal leak sensor detects drops
constantly".