PerkinElmer OilExpress 4, User Manual

Page: 63 / 152

Overview of the OilExpress 4 System . 63
7. If
Prime autosampler on initialization
was selected on the Batch Initialization dialog,
the prime starts.
Refer to
Priming the System
on page 74 for more information about priming the system.
The autosampler status is
Priming
. The spectrometer status is
Idle
.
The arm then moves to the parking position at the top-left of the deck, away from the
sample racks and tip boxes. The autosampler status is then
Idle
.
OR
If
Prime autosampler on initialization
was not selected on the Batch Initialization
dialog, then the system will perform a short prime.
The autosampler status is
Priming
. The spectrometer status is
Idle
.
The arm then moves to the parking position at the top-left of the deck, away from the
sample racks and tip boxes. The autosampler status is then
Idle
.
8. Each spectrometer collects a background spectrum.
The autosampler status is
Idle
. The spectrometer status is
Background
.
9. The autosampler moves to the first available clean tip in the first available clean tip box
and loads a disposable tip on VersaTips 1 and 3.
When starting from a full tip box, tips in position 1 and 3 (and not in position 1 and 2)
are collected.
The autosampler status is
Loading
.
10. The autosampler moves to the first two sample positions in a sample rack, and
aspirates the first two samples into the disposable tips.
Samples in positions 1 and 2 are aspirated. Each disposable tip is lowered into the
sample. Each disposable tip is lowered into the sample and the sample is aspirated by
the drawing down of the syringe.
The volume aspirated is 800 µl.
11. The autosampler then moves the disposable tips on VersaTips 1 and 3 into position over
Fill Cup 1 (which fills the lower spectrometer) and Fill Cup 2 (which fills the upper
spectrometer) of the injection station.