PerkinElmer OilExpress 4, User Manual

Page: 58 / 152

58 . OilExpress 4 System User's Guide
Overview of the FT-IR Analysis Procedure
Single-spectrometer System
NOTE: This procedure is appropriate for either a single-spectrometer system or a DUO
(dual-spectrometer) system using the lower spectrometer only. The procedure for a
DUO using the upper spectrometer only is similar, but Fill Cup 2 is connected to the
flow cell and VersaTip 4 is used for cleaning.
If you have a standard (single-spectrometer) system, the sampling procedure is as follows:
1.
Start Analysis
is selected on the Samples screen.
2. OilExpress 4 connects to the autosampler.
3.
Start
is selected on the Batch Initialization dialog.
Refer to Figure 8.
4. The fluid control module (FCM) is initialized.
The FCM initialization includes a fit-for-purpose check to ensure that it is performing
correctly.
The autosampler status is
Initializing
. The spectrometer status is
Idle
.
5. If you are using ultrasonic height sensing, then the Ultrasonic Liquid Sensor bar passes
over the sample racks, from right to left, and determines the level of liquid in each
sample.
The sensor bar then moves to the parking position at the far left of the deck, away from
the sample racks and tip boxes.
The autosampler status is
Scanning
. The spectrometer status is
Idle
.
Refer to
Ultrasonic Liquid Level Sensor Bar (Optional)
on page 54 for more information.
6. The autosampler syringes are initialized.
The syringes are initialized with the Varispan arms located over the injection station
waste trough. During syringe initialization, the syringes all move up to the same level,
pushing any system liquid already aspirated back.
The autosampler status is
Initializing
. The spectrometer status is
Idle
.
7. If
Prime autosampler on initialization
was selected on the Batch Initialization dialog,
the prime starts.
Refer to
Priming the System
on page 74 for more information about priming the system.
The autosampler status is
Priming
. The spectrometer status is
Idle
.
The arm then moves to the parking position at the top-left of the deck, away from the
sample racks and tip boxes. The autosampler status is then
Idle
.
OR
If
Prime autosampler on initialization
was not selected on the Batch Initialization
dialog, then the system will perform a short prime.
The autosampler status is
Priming
. The spectrometer status is
Idle
.
The arm then moves to the parking position at the top-left of the deck, away from the
sample racks and tip boxes. The autosampler status is then
Idle
.