PerkinElmer OilExpress 4, User Manual

Page: 62 / 152

62 . OilExpress 4 System User's Guide
24. The autosampler moves to the first available clean tip and loads a disposable tip on
VersaTips 1 and 3.
These are collected from positions 2 and 4.
25. The autosampler returns to the injection station and positions VersaTip 2 over
Fill Cup 1, and the fluid lines and flow cell are washed as in Step 13.
The autosampler status is
Cleaning
. The spectrometer status is
Idle
.
26. If the background frequency setting is set to collect a new background before every pair
of samples, a new background is collected.
The spectrometer status is
Background
.
27. The FCM pumps air through the system to dry the flow cell and the fluid lines.
The autosampler status is
Loading
.
28. As the system is dried, the autosampler moves to the sample rack and aspirates from
the next two sample positions into the disposable tips on VersaTips 1 and 3.
29. The FCM pumps the waste from samples 1 and 2 from its internal waste container out
of the Waste port on the rear of the FCM to the waste container.
Steps 9–27 repeat until all samples in the batch have been analyzed. At the end of a batch
the fluid lines and flow cell are cleaned and dried.
Dual-spectrometer (DUO) System
If you have a DUO (dual-spectrometer) system, the sampling procedure is as follows:
1.
Start Analysis
is selected on the Samples screen.
2. OilExpress 4 connects to the autosampler.
3.
Start
is selected on the Batch Initialization dialog.
Refer to Figure 8.
4. The fluid control modules (FCMs) are initialized.
The FCM initialization includes a fit-for-purpose check to ensure that it is performing
correctly.
The autosampler status is
Initializing
. The spectrometer status is
Idle
.
5. If you are using ultrasonic height sensing, the Ultrasonic Liquid Level Sensor bar passes
over the sample racks, from right to left, and determines the level of liquid in each
sample.
The sensor bar then moves to the parking position at the far left of the deck, away from
the sample racks and tip boxes.
The autosampler status is
Scanning
. The spectrometer status is
Idle
.
Refer to
Ultrasonic Liquid Level Sensor Bar (Optional)
on page 54 for more information.
6. The autosampler syringes are initialized.
The syringes are initialized with the Varispan arms located over the injection station
waste trough. During syringe initialization, the syringes all move up to the same level,
pushing any system liquid already aspirated back.
The autosampler status is
Initializing
. The spectrometer status is
Idle
.