PerkinElmer OilExpress 4, User Manual

Page: 66 / 152

66 . OilExpress 4 System User's Guide
21. The syringe dispenses system liquid from VersaTips 2 and 4 into Fill Cups 1 and 2,
respectively, and through the fluid lines for a number of wash cycles.
The wash cycles may be defined by the OilExpress 4 system (using Adaptive Cleaning)
or by the user on the Autosampler Setup dialog (Figure 45). If using Adaptive Cleaning,
the wash cycles are calculated for each sample independently.
The autosampler status is
Cleaning
.
22. If the background frequency setting is set to collect a new background before every pair
of samples, each spectrometer collects a new background spectrum.
The autosampler status is
Idle
. The spectrometer status is
Background
.
23. The FCM pumps air through the system to dry the flow cell and the fluid lines.
The air dry time is defined by the user on the Autosampler Setup dialog (Figure 45).
The autosampler status is
Loading
. The spectrometer status is
Idle
.
24. As the system is dried, the autosampler moves VersaTip 1 to the first available sample
position in a sample rack, and aspirates the next two samples into the disposable tips
on VersaTips 1 and 3.
25. Each FCM pumps the waste from the sample from its internal waste container out of the
Waste port on the rear of the FCM to the waste container.
Steps 9–24 repeat until all samples in the batch have been analyzed. At the end of a batch
the fluid lines and flow cell are cleaned and dried.