Human HumaStar 600, User Manual

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HumaStar 600 | User manual
84
6.1.3 KINETICS
Readings
Absorbance readings (A
c1
and A
c2
) for rate evaluation (consumption) are taken
at specified times (NT
c1
and NT
c2
, with values of 30 and 45 s) after last reagent
addition. Real reading times in seconds since last reagent addition are observed
(RT
c1
and RT
c2
).
Absorbance readings (A
1
to A
n
) are taken at specified times (NT
1
to NT
n
, equally
time spaced) after last reagent addition. Real reading times in seconds since last
reagent addition are observed (RT
1
to RT
n
). The number of readings is n = 10 in
normal conditions.
Once the 10 readings are taken, the linear correlation coefficient is estimated;
also, correlation is estimated but excluding all 10 points, one by one. System will
select the condition of best correlation, excluding the worst point, if necessary.
Measurement
Consumption evaluation is calculated per 1 minute as
C = (A
c2
- A
c1
) / (RT
c2
- RT
c1
) · (60 s).
The measurement is calculated as
M = b(A
i
, RT
i
) · (60 s) - B
where B is the measurement of the reagent blank if required, otherwise set to 0.
The reagent blank determination is analogous to sample reaction.
With i from 1 to n, where b(y,x) function returns the slope of the linear cor-
relation of absorbance against time pairs of values as shown in the following
equation:
where x is the represents the time and y the absorbance.
6.2 Method parameters
Several sections can be accessed in the method definition window.
6.2.1 COMMON PARAMETERS
The common parameters are available for following methods: Endpoint, Fixed
Point, Kinetics, Calculated and External.
FIGURE 85