troubleSHootInG
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3. Check for exceeded method linear range; compare method definition with
reagent specification.
4. Verify sample volume is not excessive.
5. Perform stray light verification, high range and low range linearity test, and
dilution test.
Kinetics with high dispersion or low linearity
1. Verify if incubation time is too short or heaters are not working properly.
2. Verify for abnormally high initial absorbance for decreasing kinetics (prob-
lems with reagent preparation) or too low on increasing kinetics.
Replace reagents and compare results.
3. Check lamp for stability.
4. Use new cuvettes and test again, check cuvettes for dirt or scratching.
5. Perform noise and photometric stability tests, clean filters.
6. For some kinetics: verify if sample volume is too low.
7. For some kinetics: verify centrifugation (increase time and speed).
Kinetics with normal values too high
1. Perform energy, noise and photometric stability.
2. Perform temperature verification.
3. Some kinetics: incorrect factor for selected temperature and volume.
4. Replace lamp, clean filters.
Kinetics with normal and pathological values too high
1. Verify incubation time and temperature. Perform temperature test.
2. Verify if factor matches selected temperature. Remember selected temper-
ature is usually 37°C.
Kinetics with normal and pathological values too low
1. Check for short incubation time or low temperature.
2. Verify if factor matches selected temperature. Remember selected temper-
ature is usually 37°C.
Kinetics with values too low or too high on the whole range
Verify if factor matches selected temperature. Remember selected temperature
is usually 37°C.
Summary of Contents for HumaStar 600
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