Human HumaStar 600, User Manual

Page: 86 / 132

HumaStar 600 | User manual
82
6.1.1 ENDPOINT
6.1.1.1 Single point end point
Readings
A single absorbance reading (A
1
) is taken at specified time after reagent addition.
Other absorbance reading (Ae
1
) may be taken immediately after the first read-
ing for extra precision.
Measurement
M = A
1
- B.
where B is the measurement of the reagent blank if required, otherwise set to 0.
The reagent blank determination is analogous to sample reaction.
If extra precision is required, measurement is computed as the average of the
first and extra precision readings as
M = ((A
1
+ Ae
1
) / 2) - B
Limitations
Reagent times for R2/R3 (if any) shall be 0.
6.1.1.2 Two point end point
Readings
The first absorbance reading (A
1
) is taken just before last reagent addition. The
second absorbance reading (A
2
) is taken at specified time after last reagent ad-
dition.
Measurement
The measurement is calculated as
M= (A
2
-F*A
1
) – B
Where F is a
Volume Factor Correction
given by
F= (V1 + Vs ) / (V1 + V2 + Vs)
With V
1
: first reagent volume
V
2
: second reagent volume
V
s
: sample volume