GE HEALTHCARE Biacore T100, Handbook

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Biacore T100 Software Handbook BR-1006-48 Edition AE
43
Application wizards 4
4.2.7 Prepare Run protocol
This dialog box allows you to enter a run protocol to provide instructions to the
user when the run is started. The text in the
Prepare Run Protocol is saved with
the wizard template. A suggested general protocol is provided.
Select text and use the controls at the top of the dialog box to control the
appearance (typeface, size and style) of the text.
The estimated run time and buffer requirement are shown at the bottom of the
dialog box. For all wizards except buffer scouting, only buffer A is used. For the
buffer scouting wizard (Section 4.4.2) and Method Builder-based runs
(Section 5.4), buffer names are shown in the
Prepare Run Protocol dialog.
Notes: The estimated run time is a minimum time, that does not include any time
that cannot be predicted when the wizard is set up. This includes time for
temperature equilibration at the beginning of the run or between cycles,
for ligand injection in immobilization runs using
Aim for immobilized
level (Section 4.3.2) and for conditional statements (Section 5.6.1) in
Method Builder-based runs.
The estimated buffer requirement includes a dead volume of at least 50
ml in the buffer bottle and is rounded up to the nearest 100 ml. The actual
consumption will often be considerably less than the estimate. Buffer
requirements for standby after the run (approximately 65 ml/day) are not
included.
The Menu button provides options for saving and printing the wizard template
(Section 4.2.6).