GE HEALTHCARE Biacore T100, Handbook

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4 Application wizards
4.4 Assay development wizards
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Biacore T100 Software Handbook BR-1006-48 Edition AE
4.4 Assay development wizards
4.4.1 Regeneration scouting
The Regeneration Scouting wizard guides you through the process of finding
suitable regeneration conditions for your sensor surface. The principles of
regeneration scouting are described in the Biacore Sensor Surface Handbook.
Briefly, regeneration scouting is performed by testing repeated cycles of analyte
injection and regeneration over a range of regeneration conditions, and
assessing the results on the basis of trends in analyte response and baseline
levels. The analyte concentration should be relatively high for best results. The
analyte response reflects the binding capacity (ligand activity) of the surface,
while the baseline level indicates the extent of regeneration. Each condition
should be tested for at least 3 cycles in sequence (recommended number 5) in
order to detect trends in the regeneration behavior with the given condition.
When testing multiple conditions, start with the mildest conditions to minimize
the risk of losing ligand activity at the beginning of the scouting series.
Step 1. Injection sequence
Choose the detection settings, chip type and injection sequence for the
regeneration scouting (see Section 4.2.1). One sample injection and one or two
regeneration injections are required. Most sensor surfaces can be adequately
regenerated with a single injection, but some situations may benefit from using
multiple injections.
Step 2. Setup
Specify a conditioning cycle if required.
Regeneration scouting always includes one startup cycle with the same
injection sequence as the scouting cycles but with injection of buffer for all
injections. Note that this differs from the startup cycle construction in the other
application wizards.