GE HEALTHCARE Biacore T100, Handbook

Page: 148 / 238

8 Concentration analysis
8.3 Evaluating calibration-free measurements
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Biacore T100 Software Handbook BR-1006-48 Edition AE
As a rule of thumb, exclude also samples where the reported initial binding rate
at 5 µl/min is outside the range 0.3–15 RU/s. Sensorgrams outside this range
either show too little response increase or risk insufficient mass transport
limitation for reliable evaluation.
Sensorgram display
The lower panel shows the set of sensorgrams for a selected sample. Select the
sample to display with the selector bar. By default, this panel shows data after
subtraction of blank cycles (see below). Uncheck
Show blank subtracted data
to see the unsubtracted data and the corresponding blank sensorgrams (shown
in gray).
Note: The evaluation will always use blank-subtracted data where possible,
even if this first dialog shows unsubtracted data.
Blank subtraction
Blank cycles are identified by the value y or yes in the Blank keyword (Section
B.2), and must have the same flow rate and contact time as the sample cycles
from which they are subtracted. If there is no preceding blank cycle, the nearest
following one is used. If there is no blank cycle available for one or more of the
cycles in a sample series, the evaluation will be performed using data without
blank subtraction for that series.
Click Included blanks to display an overlay plot of blank cycles in the run.
Remove the check-mark from the Include column to exclude a blank cycle from
the evaluation (for example if the sensorgram shows unreasonable response
levels or unacceptable disturbances).