GE HEALTHCARE Biacore T100, Handbook

Page: 173 / 238

Biacore T100 Software Handbook BR-1006-48 Edition AE
173
Kinetics and affinity analysis 9
Figure 9-5. For reliable evaluation, the calculated K
D
value should be less than half the
highest analyte concentration used. When this condition is met, the K
D
value is indicated
with a full black line in the result plot (left). When the condition is not met, the K
D
value is
indicated with a broken red line.
9.6 Summarizing kinetics and affinity results
You can summarize the kinetics and affinity evaluation results from several runs
or from several evaluation items for the same run using the separate Biacore
T100 Kinetics Summary software (this is installed automatically together with
the Evaluation Software).
9.6.1 Creating kinetic summaries
To create a kinetic summary of the results in a single evaluation file, simply open
the evaluation file (file type
.bme ) in the Kinetics Summary software. The
Kinetics Summary software can also be started from the Tools menu in the
Evaluation Software.
To create a summary of the results in multiple files, you can either select multiple
files within the same folder in the
Open dialog box using Ctrl-click and/or Shift-
click , or use File:Append File to add results to an existing summary. You can
open saved summary files (file type .bks) in addition to evaluation files.
9.6.2 Basic summary presentation
The summarized kinetic and affinity results are presented as thumbnail plots of
the kinetics and evaluation items and as a table of result data. Multiple fits in the
same item in the Evaluation Software are presented as separate fits in the
summary.