GE HEALTHCARE Biacore T100, Handbook

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Biacore T100 Software Handbook BR-1006-48 Edition AE
11
Introduction 1
Figure 1-1. Ligand, analyte and capturing molecule in relation to the sensor surface.
• Regeneration is the process of removing bound analyte from the surface
after an analysis cycle without damaging the ligand, in preparation for a
new cycle.
• Response is measured in resonance units (RU). The response is directly
proportional to the concentration of biomolecules on the surface.
• A sensorgram is a plot of response against time (see Figure 1-2), showing
the progress of the interaction. This curve is displayed directly on the
computer screen during the course of an analysis. Sensorgrams may be
analyzed to provide information on the rates of the interaction.
• In many assay situations, sample passes over two or more flow cells in
series, where one flow cell (usually the first) serves as a reference while
ligand is attached in the other flow cell(s). Surfaces with ligand are referred
to as
active : blank surfaces used for reference purposes are reference .
• A particular sensorgram is referred to as a curve in several contexts in the
software. This terminology is used to distinguish between different classes
of sensorgram that recur within a run: for example, measurements on one
active and one reference surface can generate separate curves for each
of the two flow cells and a third
reference-subtracted curve (active minus
reference)
• A report point records the response on a sensorgram at a specific time
averaged over a short time window, as well as the slope of the sensorgram
over the window. The response may be absolute (above a fixed zero level
determined by the detector) or relative to the response at another
specified report point.