4 Application wizards
4.3 Surface preparation wizards
44
Biacore T100 Software Handbook BR-1006-48 Edition AE
4.3
Surface preparation wizards
4.3.1
Immobilization pH scouting
The
Immobilization pH scouting
wizard helps you to find the optimal pH for
immobilizing your ligand, by testing ligand pre-concentration at a range of pH
values. See the Biacore Sensor Surface Handbook for further details. The
injection sequence for immobilization pH scouting is fixed.
Step 1. Setup
Choose the flow path for the pH scouting. Immobilization pH scouting is
restricted to a single flow cell within a run. The sensor surface in the flow cell
should be unmodified.
Enter the buffers and pH values to be used for scouting. The default list covers
sodium acetate buffers in the pH range 4 to 5.5, available as ready-to-use
solutions from GE Healthcare. Buffers will be tested in the order listed.
Note:
The buffers listed here are buffers in which the ligand should be prepared.
They are not used as running buffers: you should use the same running
buffer for pH scouting as you intend to use during immobilization.
Summary of Contents for Biacore T100
Page 1: ...GE Healthcare Biacore T100 Software Handbook ...
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Page 8: ...8 Biacore T100 Software Handbook BR 1006 48 Edition AE ...
Page 13: ...Biacore T100 Software Handbook BR 1006 48 Edition AE 13 Control Software ...
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Page 30: ...3 Manual run 3 4 Ending a manual run 30 Biacore T100 Software Handbook BR 1006 48 Edition AE ...
Page 103: ...Biacore T100 Software Handbook BR 1006 48 Edition AE 103 Evaluation Software ...
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Page 207: ...Biacore T100 Software Handbook BR 1006 48 Edition AE 207 Appendices ...
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