GE HEALTHCARE Biacore T100, Handbook

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4 Application wizards
4.3 Surface preparation wizards
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Biacore T100 Software Handbook BR-1006-48 Edition AE
Select an injection and use the Edit , Delete , Move up and Move down buttons
to edit the injection details, remove the injection from the method and change
the order of injections in the method.
Custom methods are stored in the immobilization wizard template: if you need
the same or slightly modified method in a different template, save a copy of the
template and then edit the method.
Step 2. System preparations
Check the System preparations options as required (see Section 4.2.5).
Step 3. Rack positions
Accept or change the rack positions for the various solutions required (see
Section 4.2.6).
Pre-conc injects 10 µl of ligand solution at 5 µl/min to estimate the rate of
preconcentration. This step is only performed if the option
Aim
for immobilized level is chosen when the immobilization method
is used. A method may only contain one Pre-conc injection. The
Pre-conc injection should always be placed before surface
activation: it will usually be first in the method, although it may be
preceded by a surface conditioning injection if required. If you
place the
Pre-conc injection after the surface activation, it will be
executed there and the ligand will be immobilized on the
activated surface.
After the
Pre-conc injection, the surface is washed with a solution
specified in the immobilization setup dialog, to remove any ligand
that may remain on the surface.
Do not use a
Pre-conc injection with Sensor Chip SA, since
biotinylated ligand will bind to the surface and cannot be
removed.
Inject performs an injection of a specified solution with a specified
contact time and flow rate. Values are entered in the dialog box
that appears when you click
Inject.
Mix & Inject mixes two specified solutions and performs an injection of the
mixture. Details are entered in the dialog box that appears when
you click
Mix & Inject.
Wash washes the flow system (but not the sensor surface). The wash
solution is specified in the dialog box that appears when you click
Wash.