GE HEALTHCARE Biacore T100, Handbook

Page: 161 / 238

Biacore T100 Software Handbook BR-1006-48 Edition AE
161
Kinetics and affinity analysis 9
• Report shows selected parameters and calculated values. The contents of
the Report tab are defined in the model. Global parameters are listed on a
single row at the top of the table, and local parameters are listed on one
row for each curve.
• Residuals plots the difference between the experimental and fitted curves
for each point in the curves. Use this display as an aid in judging how
closely the results fit the experimental data.
• Parameters shows the values for all parameters in the fitting equations.
Affinity results
For affinity determination, the reported K
D
value is marked on the plot as a
vertical line (for a 1:1 interaction, K
D
is the same as the analyte concentration at
a response equal to half R
max
). If the reported value is higher than half the
highest concentration used, this line will be shown broken in red as a warning
that the value may be unreliable because the plot does not flatten out
sufficiently.
The detailed results are presented on two tabs:
• Report shows selected parameters and calculated values. The contents of
the Report tab are defined in the model. Global parameters are listed on a
single row at the top of the table, and local parameters are listed on one
row for each curve.
• Parameters shows the values for all parameters in the fitting equations.