GE HEALTHCARE Biacore T100, Handbook

Page: 53 / 238

Biacore T100 Software Handbook BR-1006-48 Edition AE
53
Application wizards 4
Step 3. Injection parameters
Specify the injection parameters for each injection in the cycle (see
Section 4.2.3). The same sample will be used for all cycles.
Step 4. Experimental parameters
This dialog box determines the design of your regeneration scouting. Set the
number of conditions to test and the number of cycles for each condition and
specify the conditions in the
Settings frame. The default number of cycles for
each condition is five. You may use fewer cycles to shorten the total run time for
exploratory work, but five cycles are recommended for fine-tuning conditions in
order to reveal trends in the regeneration performance.
Use variants of the same kind of regeneration conditions (e.g. different pH
values or different concentrations of ethylene glycol) within the same run.
Results are most easily interpreted if you use a separate wizard run with a new
flow cell or sensor chip for each kind of regeneration condition that you test, so
that the outcome with one kind of condition is not affected by the history of
exposing the ligand to another.
You may choose to lock the solutions or the contact time used for regeneration
tests, so that all conditions will use the same setting for the locked parameter.
Do not vary both the solution and the contact time at the same time: the results
may be difficult to interpret clearly.
Check High viscosity solution if any of the regeneration solutions tested has a
relative viscosity higher than about 3 (corresponding to about 35% glycerol or
40% ethylene glycol at 20°C). This will modify the injection procedure for better
handling of viscous solutions.