4 Application wizards
4.2 Common wizard components
38
Biacore T100 Software Handbook BR-1006-48 Edition AE
Prime before run
This option flushes the flow system with running buffer to make sure that all
buffer is fresh. You should generally prime the system before each run to ensure
fresh buffer throughout the flow system.
Normalize
This option adjusts the detector response to compensate for small variations in
reflectance characteristics between individual sensor chips. For best results,
you should normalize the detector whenever the chip is changed. You do not
need to run normalization if the same chip remains docked between runs.
Normalization injects BIAnormalizing solution (70% glycerol) over the surface: if
your ligand does not withstand exposure to this solution, normalize the detector
before you immobilize the ligand.
Temperature settings
The
Analysis temperature
is the temperature at the flow cell. If the specified
value differs from the current temperature, the system will wait at the beginning
of the run until the analysis temperature is stable at the new value. You can
choose to ignore temperature instability, but the response will drift as the
temperature stabilizes. The absolute response decreases by about 150 RU for a
1°C increase in temperature.
The
Sample compartment temperature
is the temperature in the sample
compartment. Equilibration of the sample compartment to a new temperature
will start when the run is started. The system will not wait for a stable sample
compartment temperature at the beginning of the run: samples equilibrate to
the analysis temperature during passage through the IFC, so that the sample
compartment temperature is not critical for the measured SPR response.
Note:
Both analysis temperature and sample compartment temperature can be
set in advance with the
Set Temperature
option from the main
Tools
menu (Section 2.3.6), to allow the temperature to equilibrate before
setting up the assay wizard.
Summary of Contents for Biacore T100
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