GE HEALTHCARE Biacore T100, Handbook

Page: 143 / 238

Biacore T100 Software Handbook BR-1006-48 Edition AE
143
Concentration analysis 8
8.2.3 Samples
The Samples tab displays the measured concentrations for samples.
Sample concentrations are calculated from the nearest preceding or average
calibration curve (or the nearest following if there is no preceding curve). The
left-hand panel lists the results sorted by sample ID, with averages and CV
values for each sample ID. The column
Calc.Conc. gives the concentration
calculated for the original sample, obtained as the measured concentration
multiplied by the dilution factor. Concentrations for samples that give a
response outside the range of the calibration curve are listed as below or above
the limits of the calibration.
Note: The limits of calibration are defined by the concentrations corresponding
to the response values on the fitted curve for the highest and lowest
calibration samples. Depending on how well the curve fits the
experimental points, these limits may not coincide exactly with the actual
concentrations in the highest and lowest samples.
The right-hand panel shows the calibration curve and samples calculated from
it for the currently selected sample. Sample points represent the measured
concentrations, not the values calculated for the original samples. Select a row
in the table to highlight the corresponding point on the plot.
Right-click on a sample row in the table or a sample point on the curve and
choose
Exclude Cycle to exclude that sample from the average sample
calculation. Excluded cycles are shown as open symbols and are marked in the
table with red strikethrough text.