4 Application wizards
4.5 Assay wizards
66
Biacore T100 Software Handbook BR-1006-48 Edition AE
4.5.3
Kinetics/Affinity
The
Kinetics/Affinity
wizard guides you through the setup of experiments to
determine kinetic constants or affinity constants for an interaction. Wizards for
control experiments relevant to kinetic analysis are described in Section 4.6.
Note:
The Kinetics/Affinity wizard supports kinetic determinations in multi-cycle
format, where each analyte concentration is injected in a separate cycle
and analyte is allowed to dissociate fully or is removed by regeneration
between cycles. Another format that does not require full dissociation or
regeneration is single-cycle kinetics, supported in Method Builder (see
Section B.9).
Step 1. Injection sequence
Choose the detection settings, chip type and injection sequence for the assay
(see Section 4.2.1). Only reference subtracted detection using either Fc2-1 or
Fc4-3 is available for kinetic analysis. The Kinetics wizard supports capture but
not enhancement injections.
A
Carry Over
injection is also available in the
Kinetics/Affinity
wizard. This
performs an injection of buffer as the last injection in the sample analysis cycle,
to check that the flow system is clean from residual analyte that would
otherwise carry over to the sample injection in the next cycle. The
Carry Over
injection is fixed as a 30-second injection at 40 µl/minute, regardless of other
settings in the wizard. Report points are set automatically for the carry-over
injection to allow evaluation of potential carry-over problems.
Step 2. Setup
Summary of Contents for Biacore T100
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