GE HEALTHCARE Biacore T100, Handbook

Page: 96 / 238

5 Methods
5.7 Variable settings
96
Biacore T100 Software Handbook BR-1006-48 Edition AE
5.7 Variable settings
This screen determines whether variable parameter values are specified in the
method or at run time. You may choose to specify all values in the method, all at
run time, or a mixture of the two. Values that are specified in the method are
saved with the method and cannot be changed at run time. You can change the
level at which values are specified without changing the cycle type definition, so
that the same cycle type can be used for different assay steps with different sets
of run-time variables. An example of this may be found in the predefined
method for affinity in solution (Section B.1).
Variables are configured independently for the different assay steps (even if the
assay steps use the same cycle type).
If you choose to specify values for all variables in the method, the values are
entered in this screen. For each assay step, one row of variable values
represents one analysis cycle (the cycle may be repeated if the
Repeat property
is set in the Assay Step screen). Each row in the variables table corresponds to
a cycle in the run. A new empty row (marked with an asterisk) is created
automatically at the bottom of the table as soon as data is entered. Columns in
the table correspond to variables for the cycle type used in the assay step, and
are grouped according to commands in the cycle type definition. Use the right
Baseline Choose whether the report point will be defined as a baseline or
not. Response values for report points that are not defined as a
baseline will be calculated relative to the closest preceding
baseline value.