GE HEALTHCARE Biacore T100, Handbook

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Biacore T100 Software Handbook BR-1006-48 Edition AE
191
Kinetics and affinity analysis 9
Complex
The complex is formed on the surface and generates response and has the
properties listed below.
In the Bulk and Drift panel, enter details for bulk refractive index contribution.
Normally, there will be one bulk refractive index term applicable during
association (from the start to the end of the injection). A second term can be
used if necessary during dissociation (after the end of the injection), for example
to accommodate a permanent shift in baseline as a result of the sample
injection:
Check the Drift box and enter an expression describing the drift (most
commonly a linear function of time) to account for baseline drift.
2 Enter the reaction scheme in the Reaction panel using the pull-down list for
each reactant. Enter parameter names for the forward and backward rate
constants for each line in the reaction scheme. (The terms
k-forward and
k-backward apply to the reaction as entered in the scheme, reading from
Generates response Uncheck this box for complexes that form in
solution and that do not contribute to the response.
Molecular weight Check this box and specify a parameter for
complexes that form in solution and then bind to
the surface.
Do not check this box if
Generates response is also
checked.