GE HEALTHCARE Biacore T100, Handbook

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9 Kinetics and affinity analysis
9.9 Creating and editing models
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Biacore T100 Software Handbook BR-1006-48 Edition AE
left to right). You can also enter mathematical expressions or constant
values for the rate constants.
3 Click the Parameters tab and define the parameters used in the reaction
scheme. Click Add to add a new parameter, and define the parameter
properties in the dialog:
Choose a default type for the parameter ( Fit global , Fit local or Constant ).
For the Initial value , enter a numerical value or select a value expression
from the pull-down list. The expression represent functions evaluated
within the current data set (e.g.
Ymax is the maximum y-value in the data
set). Alternatively, choose Attach to and select a parameter from the list. If
you attach a parameter to Keyword , the initial parameter value will be set
to the value of the keyword with the same name as the parameter.
Check Allow negative value if the parameter can be below zero. Enter a
description of the parameter for ease of identification.
If you have only used single parameter names (as opposed to expressions)
for the rate constants and properties, you can click
Rate equations or OK
as a shortcut to defining parameters. The software will then enter
suggested definitions for all undefined parameters. This shortcut cannot be
used if you have entered expressions.
In the Report panel, define the parameters you want to appear in the
Report tab of the results. Report parameters are defined by a name that
may be chosen freely and a value that is entered as a parameter or
expression containing parameters.