Nano Temper Monolith NT.115 User Manual Download | Manualshive

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User Manual
Monolith

®

NT.115

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Summary of Contents for Monolith NT.115

Page 1: ...User Manual Monolith NT 115 ...

Page 2: ...terature 17 2 Monolith NT 115 Instrument 18 2 1 Models and Most Common Fluorophores 18 2 2 Application Range 18 2 3 Sensitivity 18 2 4 Sample Consumption 18 2 5 Capillary Format 19 2 6 Close to Native Conditions 19 2 7 Dedicated MO Control Software and MO Affinity Analysis Software 19 3 Preparation of MST Experiments 20 3 1 Control Kits Performing Standard Experiments 20 3 2 Selecting the Right Mo...

Page 3: ...avoided may result in minor or moderate injury It may also be used to alert against damaging the equipment or the instrument Do not proceed beyond a WARNING or CAUTION notice until you understand the hazardous conditions and have taken the appropriate steps NOTE A NOTE provides additional information to help the operator achieve optimal instrument and assay performance Read manual label This label...

Page 4: ...le invisible laser radiation Class 3B according to IEC 60825 1 2007 Lasers or laser systems emit intense coherent electromagnetic radiation that has the potential of causing irreparable damage to human skin and eyes The main hazard of laser radiation is direct or indirect exposure of the eye to thermal radiation from the infrared B spectral regions 1 400 nm 3 000 nm Direct eye contact can cause co...

Page 5: ...n the back of the chassis when the instrument is not in use CAUTION Do not use ethanol or other types of organic solvents to clean the instrument as they may remove the instrument paint CAUTION Use the instrument only for biomolecule analytics with aqueous solutions and do not open the instrument on other site than for sample loading CAUTION Only use aqueous sample for analysis in the instrument C...

Page 6: ...ls IEC 61010 2 081 2001 Safety requirements for electrical equipment for measurement control and laboratory use Part 2 081 Particular requirements for automatic and semi automatic laboratory equipment for analysis and other purposes IEC 60825 Laser safety Complies with 21 CFR 1040 10 and 1040 11 except for deviations pursuant to Laser Notice No 50 dated June 24 2007 IEC 61326 1 2006 EMC Electrical...

Page 7: ...oncondensing Operating altitude max 2000m Monolith NT 115 size Width 33 cm 13 Height 45 cm 17 5 Depth 51 cm 20 Weight 24 kg 51 lbs net Power supply size Width 10 6 cm 4 2 Height 3 cm 1 2 Depth 6 5 cm 2 6 Power supply weight 0 23 kg 0 51 lbs net max IR laser Wavelength 1475 nm 15 nm Power 120 mW max Monolith NT 115 laser classification Device is LASER PRODUCT CLASS 1 Temperature control Range 22 C ...

Page 8: ...onnecting the Ethernet cable to the PC notebook USB Connector for connecting the USB cable to the PC notebook Only used for service by NanoTemper Technologies service Power Switch When the switch is turned on position I the instrument is switched on DC Input The connector to the external power supply Connect with the external power supply ...

Page 9: ...damage incurred as a result of product use 2 Prohibitions on the use of NanoTemper Technologies software Copying software for other than backup Transfer or licensing of the right to use software to a third party Disclosure of confidential information regarding software Modification of software Use of software on multiple workstations network terminals or by other methods 3 The contents of this man...

Page 10: ...IBRATION 4 UNAUTHORIZED MODIFICATION OR MISUSE 5 USE OF UNAUTHORIZED CAPILLARIES AND CAPILLARY TRAYS 6 USE OF CONSUMABLES DISPOSABLES AND PARTS NOT SUPPLIED BY AN AUTHORIZED NANOTEMPER TECHNOLOGIES DISTRIBUTOR 7 CORROSION DUE TO THE USE OF IMPROPER SOLVENTS SAMPLES OR DUE TO SURROUNDING GASES 8 ACCIDENTS BEYOND NANOTEMPER TECHNOLOGIES S CONTROL INCLUDING NATURAL DISASTERS This warranty does not co...

Page 11: ...humidity exceeds 80 RH condensation may deteriorate optical components 9 Operate the instrument in an atmospheric pressure range of 800 1060 hPa 10 Do not operate the Monolith NT 115 under conditions which pose a risk of explosion implosion or the release of gases 11 Avoid strong magnetic fields and sources of high frequency The instrument may not function properly when near a strong magnetic fiel...

Page 12: ...at the back left of the instrument WARNING Only operate the Monolith NT 115 instrument with the external power supply provided SPU63 105 Sinpro Electronic Co Ltd or Ael60US12 XP Power LLC or GS90A12 P1M MeanWell or GS60A12 P1J MeanWell Only use the supplied cables and plugs If not doing so you risk electric shock and fire Connect the external power supply to the electrical socket Then connect the ...

Page 13: ... instrument alone two persons required for transport movement If you move the instrument alone it poses a risk of personal injury or damage to the instrument Malfunction In case of a malfunction switch off the Monolith NT 115 instrument and wait for five minutes then switch on the instrument again If the malfunction persists switch off the device unplug the power cable and contact the NanoTemper T...

Page 14: ... using e g thermal elements Changes in sample fluorescence upon activation of the IR laser are monitored to characterize and quantify binding events The response of the sample fluorescence upon IR laser activation is based on distinct physical principles which are described in the following section 1 1 1 Underlying physical principles MicroScale Thermophoresis MST is a biophysical technique that m...

Page 15: ...e after IR laser activation stems from a change in fluorescence due to TRIC and a change in the concentration of fluorophores due to MST The overall change in fluorescence after IR laser activation can thus be expressed as 𝑇 𝑐𝐹 𝐹 𝑐 𝑇 𝑐 𝐹 𝑇 Where 𝑐 𝐹 𝑇 describes changes in fluorescence due to TRIC The thermophoretic change in concentration is described by 𝐹 𝑐 𝑇 𝑆𝑇𝑐 The Soret coefficient ST describe...

Page 16: ...to locally heat a defined sample volume The MST response of fluorescent molecules within the temperature gradient are detected B Typical signal of an MST trace Initially the molecules are homogeneously distributed and a constant initial fluorescence is detected After activation of the IR laser a drop in fluorescence is observed which corresponds to TRIC triggered by the fast temperature change and...

Page 17: ...e increase to 10 C The optimal binding signal is derived based on the identification of the best signal to noise ratio at the shortest possible MST on times 1 2 Literature 1 Jerabek Willemsen M et al MicroScale Thermophoresis Interaction analysis and beyond Journal of Molecular Structure 2014 0 2 Seidel S A I et al Label Free Microscale Thermophoresis Discriminates Sites and Affinity of Protein Li...

Page 18: ...he minimum concentration for use in NT 115 series instruments can vary 2 2 Application Range The Monolith NT 115 allows measurement of the binding of virtually all biomolecules over a large range of binding affinities Kd 1 nM 500 mM 2 3 Sensitivity The Monolith NT 115 can measure as little as the binding of single ions 40 Da or small molecules 300 Da to a target as well as the binding of large com...

Page 19: ...nditions The Monolith NT 115 can monitor the binding affinity between all kinds of biomolecules under close to native conditions immobilization free in a solution of choice ranging from standard buffers to complex bioliquids including blood serum cell lysates etc 2 7 Dedicated MO Control Software and MO Affinity Analysis Software The Monolith NT 115 is supported by software for instrument control ...

Page 20: ...hnologies Monolith devices It is highly recommended to use NanoTemper Technologies capillaries for high quality and reproducible measurements NanoTemper Technologies Monolith warranty is only valid if instrument is used with NanoTemper Technologies capillaries Cat Monolith Control Kits Reactions MO C030 MonolithTM NT Control Kit RED Molecular Interaction Control Kit for MonolithTM NT 115 BLUE RED ...

Page 21: ...the optical measurement will be performed Position the liquid in the center of the Monolith Capillary by holding it vertically and shaking it after aspirating the sample Cat Monolith Protein Labeling Kits Reactions MO L001 MonolithTM Protein Labeling Kit RED NHS Amine Reactive 4x 100 µg protein MO L002 MonolithTM Protein Labeling Kit GREEN NHS Amine Reactive 4x 100 µg protein MO L003 MonolithTM Pr...

Page 22: ... the front of the tray This position is denoted as 1 on the sample tray and in the control software Place the sample tray in the instrument by pushing it into the tray slot of the instrument as far as possible The Monolith NT 115 instrument scans the tray and automatically determines the position of the capillaries on the tray ...

Page 23: ...espectively The status of the LED informs you if fluorescence detection is running and the status of the MST power shows if the IR laser is working and generating temperature gradients in the samples In the middle of the screen you find information on the Temperature Control First you can see if the Temperature Control is activated OFF or ON this is set with the control software Below this the Act...

Page 24: ...one 49 0 89 4522895 0 Fax 49 0 89 4522895 60 info nanotempertech com http www nanotempertech com MicroScale Thermophoresis is a trademark NanoTemper and Monolith are registered trademarks NanoTemper and Monolith are registered in the U S Patent and Trademark Office V24_2018 04 05 ...

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