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8 Troubleshooting | 8.3 Chamber
ZEISS
8.3.4 Replacing the Chamber Door Seal
Possible reasons for replacing the chamber door seal are the following:
§
Chamber door does not close tightly
§
Bad chamber vacuum
Overview
The procedure contains the following steps:
§
Venting the Specimen Chamber [
§
§
Evacuating the Specimen Chamber [
8.3.4.1 Replacing the O-ring
WARNING
Suffocation hazard: Lack of oxygen
Gaseous dry nitrogen is used to vent the specimen chamber during specimen exchange. Inhal-
ing nitrogen may cause unconsciousness.
4
During specimen exchange, keep the chamber door open as short as possible.
4
Do not inhale the air from within the specimen chamber.
4
Ensure that the area around the microscope is sufficiently vented.
4
If you begin to experience symptoms of asphyxia (for example: rapid breathing, loss of
mental alertness and/or muscular coordination, depression of sensations, emotional insta-
bility, fatigue) leave the room immediately and inform the facility’s safety officer.
NOTICE
Risk of property damage: Contamination caused by fingerprints
Contamination caused by fingerprints can lead to vacuum deterioration or prolonged pumping
times.
4
Always wear lint-free gloves when touching the specimen, specimen holder, or stage.
1. Carefully open the chamber door.
2. On the inside of the chamber door, remove the chamber door O-ring.
NOTICE
Risk of property damage: If you use a metal tool to remove the O-ring,
then you may damage the sealing surface. If necessary, then only use a plastic or
wooden tool to remove the O-ring.
3. Inspect the groove that holds the O-ring and remove any contamination.
4. Insert the new chamber door O-ring.
5. Close the chamber door.
8.3.5 Resetting the Touch Alarm
To prevent damage, a touch alarm is integrated in the microscope. If the specimen or the speci-
men holder touches the chamber walls, the detectors, or the objective lens, the stage is stopped
immediately. An audible warning sounds and an on-screen message is displayed.
Prerequisite
ü
The EM Server shows the message
WARNING Stage Touching
.
1. To accept the warning, click
OK
.
2. Move the stage in the reverse direction away from the touch.
100
Instruction Manual ZEISS Crossbeam 350 | en-US | Rev. 3 | 349500-8111-000