Nikon Eclipse Ni-U Instruction Manual Download

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Upright Microscope

Instruction Manual

Assembly/Maintenance

M570 E

11.8.NF.1 (2/2)

Summary of Contents for Eclipse Ni-U

Page 1: ...Specifications Maintenance and Storage Troubleshooting Assembly Upright Microscope Instruction Manual Assembly Maintenance M570 E 11 8 NF 1 2 2 ...

Page 2: ......

Page 3: ...nsure the accuracy of this manual errors or inconsistencies may remain If you note any points that are unclear or incorrect please contact your nearest Nikon representative Some of the equipment described in this manual may not be included in the set you have purchased If you intend to use any other equipment with this product read the manual for that equipment too If this equipment is used in a m...

Page 4: ...ly Maintenance This manual Assembly Troubleshooting Maintenance and Storage Specifications Before reading the Assembly Maintenance manual read the Safety Precautions in the Operation manual Symbols Used in This Manual The following symbols are used in this manual Symbols for Safety WARNING CAUTION Highlights important information that should be noted for safety Read Safety Precautions for details ...

Page 5: ...Maintenance and Storage Troubleshooting Assembly Summary of Contents See the next page for the detailed contents Introduction Contents of the Manual Symbols Used in This Manual Assembly Troubleshooting Maintenance and Storage Specifications ...

Page 6: ...ser holder 12 12 Attach the stage 12 12 1 For standard stage 12 12 2 For rotatable ceramic coated stage 12 13 Attach the condenser 13 Attaching the optical module to the universal condenser 13 Attaching the DIC module to DIC condenser oil 17 14 Attach the nosepiece 19 15 Attach the objective 19 16 Attach the DIC rotation polarizer unit required for differential interference contrast microscopy 19 ...

Page 7: ...torage 35 1 Replacing the Lamp 36 2 Cleaning 37 2 1 Cleaning Lenses 37 2 2 Cleaning Parts Other than the Lens 37 2 3 Cleaning Immersion Oil 38 2 4 Decontaminating the Product 38 3 Transportation 38 4 Storage 38 5 Regular Inspections Charged 38 Specifications 39 1 Microscopy Principles 40 2 Performance Properties 40 3 Physical Properties 43 ...

Page 8: ...Contents vi ...

Page 9: ... notes such as CAUTION 9 Cautions on assembling and installing the product at the beginning of the separately provided instruction manual Operation or 4 Installation location in Notes on Handling the Product in the same manual before you work on assembling Assembly tools provided with the microscope Hex driver x3 2 mm across flats x3 Hex wrench x2 3 and 4 mm across flats ...

Page 10: ...ort Quadrocular tilting tube Camera cable to DS U3 or DS L3 Motorized shutter cable to control box B Motorized shutter Motorized shutter adapter Contact arm Standard arm Lambda plate DIC slider DIC nosepiece 1 To control box B Filter cube FL DIC analyzer Excitation balancer RS 232C cable to control box B for motorized operation Motorized shutter cable to control box B Motorized shutter adapter Mot...

Page 11: ...ser 1 100x C C phase turret condenser Cannot be used when rotatable ceramic coated stage is attached DIC condenser Oil D CUO Used with OIL DIC module Condenser Universal condenser Dry NI CUD Supports DRY DIC module PH module dark field module NI CALN1 2 4x auxiliary lens D C 2 4x auxiliary lens Cube turret NI FLT6 Intelligent epi fluorescence cube turret NI FLT6 I Epi fluorescence cube turret Moto...

Page 12: ...ent attached on the microscope setup menu for the DS L3 DS Camera Control Unit See Chapter 3 19 Operations on DS L3 19 1 Setting Up the Microscope 1 Check the input voltage Check the input voltage indicated on the back of the microscope Use the microscope only if the indicated input voltage matches the power supply voltage for the area in which the microscope will be used WARNING If the indicated ...

Page 13: ... cable to the LAMP connector on the rear of the main body DSC LAMP CTRL BOX B LAMP DC12V 100W MODEL ECLIPSE Ni U NIKON CORP ORAT ION TOKY O JAPA N 100 2 40V 1 7A 50 60H z MADE IN JAPAN This device complie s with Part 15 of the FCC Rules Operati on is subject to the followin g two conditio ns 1 this device may not cause harmful interfere nce and 2 this device must accept any interfere nce receive d...

Page 14: ...beforehand Tool Hex wrench 4 mm across flats 1 Place the arm while aligning it with the two positioning pins on the main body and tighten the four fixing screws 2 When using contact arm connect the cable from the CONTACT ARM1 connector to the CONTACT ARM1 connector on the control box B 3 Connect the cable from the CONTACT ARM2 connector to the CONTACT ARM2 connector on the control box B Standard a...

Page 15: ...epi fluorescence cube turret 5 Tighten the fixing screw loosened in step 3 6 Tighten the four fixing screws on top of the epi fluorescence attachment 7 Attach the HG adapter to the bayonet mount at the rear of the epi fluorescence attachment and connect the HG precentered fiber illuminator See the instruction manual provided with the HG precentered fiber illuminator F STOP A STOP EX ADJ Connecting...

Page 16: ...ch the filter cube to the slot 3 Insert the filter cube nameplate into the slot cover window Insert into the same address window as the address shown inside the slot 5 6 1 A λ 6 1 5 4 3 2 1 ND4 ND8 ND16 A 1 2 3 5 UV 2A EX 330 380 DM 400 BA 420 4 Turn the cube switchover turret to attach the filter cubes in the remaining slots and also insert the nameplates For bright field microscopy For bright fi...

Page 17: ... outer frame Make sure that the arrow faces the direction of a dichroic mirror when attaching the filter Contact your nearest Nikon representative when the dichroic mirror needs to be replaced UV 2A EX 330 380 DM 400 BA 420 Replacing the excitation and barrier filters Attaching a light shielding plate Tool Hex driver 2 mm across flats Lightly screw the two fixing screws provided with the light shi...

Page 18: ...Loosen the fixing screws x2 on both sides of the zooming port with a hex driver 2 mm across flats 5 Align the round dovetail of the quadrocular tube with the round dovetail of the arm and slide the entire attached devices to the rear 6 Tighten the fixing screw loosened in step 3 7 Tighten the fixing screws x2 on top of the zooming port with a hex wrench 3 mm across flats 8 Tighten the fixing screw...

Page 19: ...ed The procedure is the same for both models Tools Hex driver 2 mm across flats Hex wrench 3 mm across flats 1 Place the square groove on the substage onto the reference guide bar on the elevating section of the main body Move the substage to the left press it against the reference surface of the elevating section and tighten the drop proof fixing screws x3 with hex wrench 3 mm across flats 2 Put ...

Page 20: ...ge 12 2 For rotatable ceramic coated stage Tools Hex driver 2 mm across flats Centering tool Ball point hex drivers provided with the product x2 1 Sufficiently loosen the two right and left centering screws on the back of the substage and the stage positioning screw on the front of the stage using a hex driver 2 Attach by engaging the round dovetail at the bottom of the stage with the round doveta...

Page 21: ...jective See following table If the combination is not correct differential interference contrast image cannot be obtained or the contrast decreases significantly To support for particular purposes module for higher contrast or resolution are available Note however that in principle the contrast contradicts the resolution of the differential interference contrast image the higher the contrast the l...

Page 22: ...C PH 1 2 or 3 PH module D C PH 2 4 PH module D C PH 3 DIC module D C DIC N2 DRY DIC module D C DIC NR DRY Dark field module D C DF 5 or 6 2 4x auxiliary lens D C 2 4x φ39 DIC module D C DIC N1 DRY 7 2 4x auxiliary lens NI CALN1 φ35 Module replacement cover Replacement cover fixing screw two Replacement cover removed Hole number PH module centering knob Use the centering knob by pulling it out hori...

Page 23: ...ew and tighten the module fixing screw to secure After it is tightened pull out the knob and restore it to the original position Attaching PH module Holes 2 3 and 4 have a spring First check that the tips of the two Ph module centering screws are not inside the hole If it is inside place that hole into the optical path lift the Ph module centering knob at right and left rear of the condenser and t...

Page 24: ...luor 20X MI Plan Apo 20X Plan Apo VC 20X Plan Apo λ 20X S Fluor 20X Fluor 20X W 20x 20X C Plan Fluor 40X Plan Apo 40X Plan Apo λ 40X S Fluor 40X Apo LWD 40X WI λS 40X I N1 Dry 40X I C Plan Fluor 40X Oil S Fluor 40X Oil Apo 40X WI λS 40X II 40x Fluor 40X W Apo 40X W NIR 40X III Plan Apo VC 60X H Plan Apo 60X Plan Apo λ 60X Fluor 60X W Apo TIRF 60X Oil Apo 60xW NIR 60X I 60X I R Plan Fluor 60X Oil P...

Page 25: ...ch the DIC module to the slider Identify the slider for the DIC module by the prism name sticker N2 and NR affixed to the handle and attach the DIC module to it Align the module orientation groove on the bottom of the DIC module with the slider s orientation pin and tighten the DIC module fixing screw on the slider Attaching the DIC module 2 Insert the DIC module slider into the condenser For the ...

Page 26: ...0X Plan Apo λ 20X S Fluor 20X Fluor 20X W 20x Plan Fluor 40X Plan Apo 40X Plan Apo λ 40X S Fluor 40X Apo LWD 40X WI λS 40X I Plan Fluor 40X Oil S Fluor 40X Oil Apo 40X WI λS 40X II 40x Fluor 40X W Apo 40X W NIR 40X III Plan Apo VC 60X H Plan Apo 60X Plan Apo λ 60X Fluor 60X W Apo TIRF 60X Oil Apo 60xW NIR 60X I 60X I R Plan Fluor 60X Oil Plan Fluor 60X A Plan Apo λ 60X Oil Apo 60X H λS 60X II 60X ...

Page 27: ... when the nosepiece is rotated clockwise when viewed from the top 16 Attach the DIC rotation polarizer unit required for differential interference contrast microscopy Tool Hex driver 2 mm across flats 1 Loosen the polarizer rotation clamp screw rotate the polarizer to align the indicator lines and then clamp it 2 Put the rotatable polarizer unit over the field lens on the microscope base 3 Use the...

Page 28: ...on connector for the camera head and the camera connector for the DS L3 DS U3 DS camera control unit using a provided camera cable 5 Connect the EXT I O connector for DS L3 DS U3 with the DSC connector for the microscope or the Ex OUT connector for the connector box B using a camera trigger cable For DS L3 a USB cable can also be used for connecting the USB H connector for DS L3 and the USB connec...

Page 29: ...mpletely Prior to photomicrography adjust the camera position and adjust the focus on the monitor as appropriate See Chapter 3 18 Capturing Images Photomicroscopy in the Operation Attaching a camera to the DSC zooming port for quadrocular tube 18 3 When attaching to the quadrocular tilting tube Tool Hex driver 2 mm across flats Attach the tube adapter for quadrocular tube to the quadrocular tiltin...

Page 30: ...pment are turned off before connecting the cable When the microscope is motorized see the figure on next page and connect the cable correctly To connect the LAMP CTRL BOX B on the microscope to the LAMP CTRL Ni U on the control box B the lamp control cable is required To connect the DSC on the microscope to the DSC Ni U on the control box B the C CTC camera trigger cable L3 U3 is required To conne...

Page 31: ...dy rear CONTACT ARM1 DIA SHUTTER EPI SHUTTER 100 240V 1 0A 50 60Hz CONTACT ARM2 DSC Ni U Ex OUT HGFIE REMOTE USB LAMP CTRL Ni U Control box B rear DS L3 DS camera control unit CONTACT ARM1 Connect to CONTACT ARM1 on control box B DSC Connect to DSC Ni U on control box B or EXT I O on DS L3 U3 CONTACT ARM2 Connect to CONTACT ARM2 on control box B LAMP Connect to dia illumination lamphouse AC inlet ...

Page 32: ...g the power cord into the AC inlet on the microscope main body 3 Plug the other end of each power cord into a wall outlet Connecting the power cord to the connected motorized device Check that the power switch for control box B or DS camera control unit is turned off plug the power cord into the AC inlet and then plug the other end to the wall outlet ...

Page 33: ...s properly functional If any of the problems described in this chapter occurs be sure to check the table for possible causes before requesting service If you detect problems that are not listed in the table or the problem still persists after measures are taken turn off the device and contact your nearest Nikon representative ...

Page 34: ... too far 5 Open it to proper size See Chapter 3 6 Adjusting the Aperture Diaphragm in the Operation Dirt or dust on the monitor moves when the camera is turned Lens or specimen is dirty or dusty Check and clean it in accordance with the Dirt or dust does not rotate when eyepiece is turned of Dirty or dusty field of view when looking into eyepiece Dirt or dust on the monitor does not move when the ...

Page 35: ...s of a Diascopic Image in the Operation The lamp voltage is too low Turn the brightness control knob to the mark position and adjust the brightness with the ND filters See Chapter 3 1 Adjusting the Brightness of a Diascopic Image in the Operation The condenser aperture diaphragm is stopped down too far This should normally be adjusted to 70 to 80 of numerical aperture of the objective See Chapter ...

Page 36: ...Assembly Maintenance Lack of visibility around periphery of field of view Illumination is uneven across the field of view Field of view is not visible The lens and specimen are dirty or dusty Clean them as appropriate See Chapter 3 2 1 Cleaning Lenses in the Assembly Maintenance The specimen is upside down Turn up the cover glass and attach it to the stage See Chapter 2 2 Bright Dark field Microsc...

Page 37: ...are not coincident Diopter adjustment has not been performed Perform diopter adjustment See Chapter 3 4 Adjusting the Diopter in the Operation Diopter adjustment has not been performed Perform diopter adjustment See Chapter 3 4 Adjusting the Diopter in the Operation Eyes become fatigued Brightness is inadequate Adjust the brightness using the brightness control knob or ND filters to attain a suita...

Page 38: ...lters and the Aperture Diaphragm in the Operation A halogen light source is used for a dark specimen Change the light source to a mercury lamp A designated objective is not used at UV or V excitation Use a designated objective The fluorescent image is very dark when the lamp is ON The room is bright Make it darker The dia illumination lamp is on Turn off the dia illumination lamp The filter cube b...

Page 39: ...ntrast Microscopy 14 Adjust the orientation vibration direction of the polarizer and analyzer in the Operation The analyzer is out of the optical path Bring the analyzer into the optical path See Chapter 2 4 Differential Interference Contrast Microscopy 14 Adjust the orientation vibration direction of the polarizer and analyzer in the Operation A correct DIC module on the condenser has not been se...

Page 40: ...ge is not focused on the specimen surface Make sure the condenser is focused and centered See Chapter 3 5 Focusing and Centering the Condenser in the Operation Poor contrast The immersion oil is not sufficient in the oil condenser and air bubbles are between the slide and the condenser Apply the immersion oil again See Chapter 3 12 Oil Immersion Water Immersion in the Operation 1 4 Phase Contrast ...

Page 41: ...ct combination See Chapter 1 3 Assembly Method Introduction in the Assembly Maintenance Illumination There is no power supplied Plug in the power cord See Chapter 1 3 Assembly Method 21 Connect the power cord in the Assembly Maintenance The lamp has burned out Replace the lamp with the specified type See Chapter 3 1 Replacing the Lamp in the Assembly Maintenance Lamp does not light The lamp is not...

Page 42: ...or s manual The mercury lamp does not work The mercury lamp s connector is not connected to the illuminator Connect it to the power supply Check your illuminator s manual The mercury lamp burns out soon after it is turned on The lamp type is incorrect The lamp is at end of its life Replace the lamp with the specified type Check your illuminator s manual Motorized epi fluorescence cube turret Probl...

Page 43: ...35 Maintenance and Storage 3 Chapter 3 Maintenance and Storage This chapter describes how to replace the halogen lamp and how to clean and store the product ...

Page 44: ...t Used lamps Do not break used lamps It should be disposed of as industrial waste according to local regulations and rules Tool Hex wrench 3 mm across flats 1 Loosen the lamphouse cover fixing screw and lift up the cover to remove 2 Hold down the lamp clamp lever and remove the old lamp 3 Attach a new lamp Avoid touching the glass surface of the lamp with your bare hands Attach the new lamp while ...

Page 45: ...ne should be used only to wipe off immersion oil from the objective and never to clean the entrance lens at the bottom of the eyepiece tube prism surface of the eyepiece tube or the filters 2 1 Cleaning Lenses Keep the lens free of dust and fingerprints If there is contamination on the lenses or filters image quality decreases If any of the lenses become dirty clean them by following the procedure...

Page 46: ...devices be sure to fasten the devices Manual quadrocular tilting tube Attach the tightening screw removed from the bottom of the tube in Chapter 1 3 Assembly Method 7 Attach the quadrocular tilting tube and DSC zooming port for quadrocular tube Set the optical path switching dial to the F position in advance Manual DSC zooming port Set the zoom dial to 0 6x no tightening screw 4 Storage Store this...

Page 47: ...39 Specifications 4 Chapter 4 Specifications ...

Page 48: ...ser It is intended for the researchers medical professional and those who work on experiments in the field of pathology and cytology 2 Performance Properties Nikon Microscope ECLIPSE Ni U Model ECLIPSE Ni U Main body Optical system Infinity corrected CF optical system Objective CFI60 Eyepiece Field number 22 when using ergonomic tube or binocular tube Field number 25 when using trinocular tube T F...

Page 49: ...stage can be rotated clockwise by 195 and counterclockwise by 7 from the reference position rotation angle 0 Condenser holder Equipped with condenser focus knob condenser centering screws Attached to a substage Condenser Universal condenser Attach optical modules for differential interference contrast phase contrast or dark field microscopy to a turret C C phase turret condenser Optical module for...

Page 50: ...nding 3 conductor grounding Type SVT No 18 AWG 3 m long maximum rated at 125 VAC minimum When used in 220 240 V regions Detachable power cord set approved according to EU EN standard 3 conductor grounding 3 conductor grounding Type H05VV F 3 m long maximum rated at 250 VAC minimum When used inside Japan PSE approved detachable power cord set 3 conductor grounding 3 conductor grounding Type VCTF 3 ...

Page 51: ...ence Cube Turret NI SH E Motorized Shutter C HGFIE HG Precentered Fiber Illuminator This product meets FCC Part 15B Class A requirements This equipment has been tested and found to comply with the limits for a Class A digital device pursuant to Part 15 of the FCC Rules These limits are designed to provide reasonable protection against harmful interference when the equipment is operated in a commer...

Page 52: ...tion External dimensions and weight External dimensions 93 W x 160 H x 220 D mm excluding projections Weight 2 2 kg Simple Remote Control Pad for Nikon Microscope Model NI SRCP Operating conditions Temperature 0 C to 40 C Humidity 60 RH max no condensation Altitude 2000 m max Pollution degree Degree 2 Installation Category II Electrical shock protection class Class I Indoor use only Transport stor...

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