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ZEISS
6 Operation | 6.2 Obtaining a First Image
6.2.2.4 Mounting the Specimen Holder
WARNING
Suffocation hazard: Lack of oxygen
Gaseous dry nitrogen is used to vent the specimen chamber during specimen exchange. Inhal-
ing nitrogen may cause unconsciousness.
4
During specimen exchange, keep the chamber door open as short as possible.
4
Do not inhale the air from within the specimen chamber.
4
Ensure that the area around the microscope is sufficiently vented.
4
If you begin to experience symptoms of asphyxia (for example: rapid breathing, loss of
mental alertness and/or muscular coordination, depression of sensations, emotional insta-
bility, fatigue) leave the room immediately and inform the facility’s safety officer.
CAUTION
Risk of injury: Moving the specimen stage
Fingers can be trapped by the moving specimen stage.
4
Always close the chamber door before moving the specimen stage.
4
To remove parts fallen into or near to the stage use a tool (e.g. tweezers) instead of your
fingers.
CAUTION
Risk of injury: Closing the chamber door
Fingers can be pinched when closing the chamber door.
4
Use the door handle to close the chamber door.
4
Ensure not to get your fingers caught in the chamber door gap.
NOTICE
Risk of property damage: Short working distance
When opening the chamber door, the microscope or specimen can be damaged if the speci-
men stage is at a short working distance. If a BSD detector is inserted, it can be damaged as
well.
4
Always move the specimen stage to a long working distance before opening the chamber
door.
NOTICE
Risk of property damage: Contamination caused by fingerprints
Contamination caused by fingerprints can lead to vacuum deterioration or prolonged pumping
times.
4
Always wear lint-free gloves when touching the specimen, specimen holder, or stage.
Instruction Manual ZEISS Crossbeam 350 | en-US | Rev. 3 | 349500-8111-000
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