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5-4 Operator’s Manual – OPTI CCA-TS
5 SAMPLE HANDLING AND PATIENT TESTING
5.1.8 Test Conditions
Sample Size: a minimum of 125 µL (60µL for B60 cassette)
Sample Type: heparinized whole blood, serum, plasma
Sample Application: syringe, capillary or ComfortSampler
Ambient Temperature: 10 - 32 ºC (50 – 90 ºF)
Relative Humidity: 5% to 95% (non-condensing)
Type of Measurement: optical fluorescence (pH, PO
2
, PCO
2
, Na
+
, K
+
, Ca
++
, Cl
-
,
Glu, BUN (urea), Lac), and reflectance (tHb, SO
2
)
5.2 Sample Preparation
5.2.1 Whole Blood Samples
Collect blood in a heparinized syringe, a capillary tube or a ComfortSampler.
Whole blood samples should be analyzed as soon as possible, ideally within 5 minutes after
collecting the sample. For brief storage of up to one hour, the sample should be iced.
WARNING: Whole blood samples require the proper amount of anticoagulant to prevent the
sample from clotting. DO NOT use anticoagulants such as EDTA, citrate, oxalate,
etc. Use only heparin salts as anticoagulants.
WARNING: Sedimentation of red cells may occur rapidly in whole heparinized blood.
This may affect your tHb results. Make sure your sample is free of trapped gas
bubbles and completely mixed, by rolling the syringe between the palms of your
hands and inverting end over end for at least one minute, just prior to sample
introduction.