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Operator’s Manual – OPTI CCA-TS A-9
APPENDIX A - TECHNICAL SPECIFICATIONS
HCO
3
-
Bicarbonate concentration in plasma.
HCO CO
3
pH − −
= ⋅ ⋅ 0 0307 10
2
6 105
.
( . )
P
[mmol/L]
1
st.HCO
3
-
Standard bicarbonate of the blood, defined as the plasma bicarbonate concentration in blood
which has been equilibrated at 37 ºC with a gas mixture having a P CO
2
= 40 mmHg.
( )
st.HCO 10
3
st.pH 6.022 − −
=
[mmol/L]
6
tCO
2
Total concentration of CO
2
in plasma, the sum of dissolved CO
2
and bicarbonate.
tCO HCO (0.0307 CO )
2 3 2
= + ⋅ − P
[mmol/L]
7
BE
The base excess of the blood results from a calculation to determine the titratable base of the
blood, which in principle is measured by titration of the blood with a strong acid or base to a pH
of 7.4 with P CO
2
= 40 mmHg at 37 ºC.
( )( )
[ ]
−
+ − − ⋅ + ⋅ ⋅ ⋅ − =
3
HCO 24.8 7.4 pH 7 . 7 tHb 43 . 1 tHb) 0.014 (1 BE
[mmol/L]
7
BE
ecf
The base excess of extracellular fluid is a quantity that reflects only the non-respiratory
components of acid-base balance (tHb = 5 g/dL).
( )
−
+ − − ⋅ =
3 ecf
HCO 8 . 24 4 . 7 pH 2 . 16 BE
[mmol/L]
7
7
CLSI. Blood Gas and pH Analysis and Related Measurements; Approved Guideline. NCCLS document C46-A,
2001.